Abstract Hydrogel is an ideal material state for cell encapsulation. However, its biomechanical properties and cell growth are inversely proportional in 3D bioprinting applications. Here, we prepared a fiber-reinforced gelatin-methacrylate (gelMA) hydrogel bioink that can improve the compressive modulus and cell proliferation using poly(lactic-co-glycolic acid) n anofiber f ragments (PLGA-NF) as an artificial extracellular matrix to build patient specific soft tissues. The microstructures of the nanofiber fragments were observed via scanning electron microscopy. The degree of substitution of synthesized gelMA was investigated considering the mole ratio of MA and gelatin. The compressive modulus of hydrogels was measured using a texturometer. Cell viability of the encapsulated fibroblast was confirmed using live-dead stains. The morphology of actin, cytoskeleton, and nucleus of cells in gelMA/PLGA-NF bioinks were analyzed using phalloidin-DAPI stains. The viscosity and compressive modulus of the hydrogel were found to have significantly increased by the incorporation of 1 wt% PLGA-NF. Cell proliferation in bioprinted gelMA/PLGA-NF hydrogels was significantly increased in comparison to the control groups. The combination of hydrogel bioink with nanofibrous fragments will be a critical footstep in the field of 3D bioprinting.

中文翻译：

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用于 3D 生物打印的含有聚（乳酸 - 共 - 乙醇酸）纳米纤维片段的增强明胶 - 甲基丙烯酸酯水凝胶

摘要 水凝胶是一种理想的细胞包封材料状态。然而，它的生物力学特性和细胞生长在 3D 生物打印应用中成反比。在这里，我们制备了一种纤维增强的明胶 - 甲基丙烯酸酯（gelMA）水凝胶生物墨水，它可以使用聚（乳酸 - 共 - 乙醇酸）纳米纤维片段（PLGA-NF）作为人工细胞外基质来提高压缩模量和细胞增殖。构建患者特定的软组织。通过扫描电子显微镜观察纳米纤维碎片的微观结构。考虑到MA与明胶的摩尔比，研究了合成gelMA的取代度。使用质地计测量水凝胶的压缩模量。使用活-死染色确认封装的成纤维细胞的细胞活力。肌动蛋白的形态，使用鬼笔环肽-DAPI 染色剂分析gelMA/PLGA-NF bioinks 中的细胞骨架和细胞核。发现水凝胶的粘度和压缩模量通过掺入 1 wt% PLGA-NF 显着增加。与对照组相比，生物打印的 gelMA/PLGA-NF 水凝胶中的细胞增殖显着增加。水凝胶生物墨水与纳米纤维碎片的结合将成为生物 3D 打印领域的关键一步。