Interest in medicinal and aromatic plants as a source of anti-microbial drugs has emerged, mainly due to increased resistance in pathogenic moulds. The aims of this study were to evaluate the prevalence and factors influencing the presence of onychomycosis in immunocompromised patients as well as to assess the anti-fungal activity of Zataria multiflora, Origanum vulgare, Heracleum persicum, Rosmarinus officinalis, Pimpinella anisum, Thymus kotschyanus and Artemisia sieberi essential oils against nondermatophytic moulds. During the period of 5 years (2012–2017), a total of 379 patients with suspected onychomycosis were examined for mould etiologic agents, with mycological examinations. The minimum inhibitory concentrations (MICs) of essential oils were determined based on the Clinical and Laboratory Standards Institute (CLSI, M38-A) protocol. Among 379 subjects, onychomycosis due to moulds was confirmed in 145 cases (83 males and 62 females, aged 20–80 years). The most frequent moulds isolated were Fusarium solani (F. solani), followed by Scopulariopsis brevicaulis (S. brevicaulis), Acremonium spp., Aspergillus fumigatus (A. fumigatus), F. oxysporum, A. flavus and Cladosporium spp. All isolated moulds were sensitive to the essential oils, ranging from 20 to 530 μg/mL. The MIC values ranged from 20 to 50 μg/mL for Zataria multiflora, 20–60 μg/ml for Origanum vulgare, 40–250 μg/ml for Thymus kotschyanus, 20–340 μg/ml for Artemisia sieberi, 50–490 μg/ml for Pimpinella anisum, 85–410 μg/ml for Rosmarinus officinalis and 80–530 μg/ml for Heracleum persicum. The results confirmed the importance of nondermatophyte moulds in fungal nail and toenail infections and efficiency of the essential oils, in different ranges, against the moulds obtained from onychomycosis patients.

人们对药用和芳香植物作为抗微生物药物的兴趣已经出现，这主要是由于病原体霉菌的抗药性增强。这项研究的目的是评估的流行和影响灰指甲的存在免疫功能低下患者，以及评估的抗真菌活性的因素Zataria野蔷薇，牛至，独活梭梭，迷迭香，茴芹，百里香kotschyanus和蒿西贝里对抗非皮肤真菌的精油。在5年（2012-2017年）期间，共对379名疑似灰指甲病患者进行了霉菌病原学检查，并进行了真菌学检查。根据临床和实验室标准协会（CLSI，M38-A）协议确定精油的最低抑菌浓度（MIC）。在379名受试者中，证实有145例由霉菌引起的灰指甲病（男性83例，女性62例，年龄20-80岁）。分离的最常见的霉菌是镰孢镰刀菌（F. solani），其次是短角S（Scopulariopsis brevicaulis）（S. brevicaulis），顶孢菌属（Acremonium spp。），烟曲霉（Aspergillus fumigatus）（A. fumigatus）。），F.oxysporum，A.flavus和Cladosporium spp。所有分离的霉菌均对精油敏感，范围为20至530μg/ mL。的MIC值范围为20至50微克/毫升为Zataria野蔷薇， 20-60微克/毫升为牛至，40-250微克/毫升为胸腺kotschyanus，20-340微克/毫升为蒿sieberi，50-490微克/毫升茴芹，85-410微克/毫升为迷迭香和80-530微克/毫升为独活仙客来。 结果证实了非皮肤癣菌霉菌在真菌指甲和趾甲感染中的重要性以及针对从甲癣患者获得的霉菌在不同范围内的精油功效。