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Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non-refrigerated raw chicken livers.
International Journal of Food Microbiology ( IF 5.0 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.ijfoodmicro.2020.108669
Said Kamal Abolghait 1 , Amal Gamal Fathi 2 , Fatma Mohamed Youssef 2 , Abdelazeem Mohamed Algammal 3
Affiliation  

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections and staphylococcal food poisoning (SFP). This study was conducted to investigate the prevalence and enterotoxigenicity of MRSA in broiler chicken meat and giblets. A total of 5.5% (8/144) of the examined samples were contaminated with mecA positive/mecC negative MRSA, with staphylococcal counts of approximately 102 colony forming units (CFU)/g in breast, leg and gizzard samples and approximately 3.3 × 103 CFU/g in frozen liver samples. Most MRSA isolates (75%, 6/8) harboured the staphylococcal enterotoxin B (seb) gene. Reverse transcription-PCR (RT-PCR) showed that MRSA isolates initiated SEB production in experimentally contaminated chicken livers within 24 h of storage at temperatures over 8 °C. SEB was maximally produced at 24 °C when the MRSA counts reached 7.3 × 103 ± 1.2 × 103 CFU/g sample homogenate. The current study concludes that the main broiler chicken MRSA isolates in Egypt harbour the seb gene. To mitigate possible SEB production, especially in broiler chicken livers, a maximum “out of refrigeration” time limit should be implemented for cold chain poultry products.



中文翻译:

从鸡肉和内脏中分离出的耐甲氧西林金黄色葡萄球菌(MRSA)通常在未冷藏的未加工鸡肝中产生葡萄球菌肠毒素B(SEB)。

耐甲氧西林金黄色葡萄球菌(MRSA)导致几种难以治疗的感染和葡萄球菌食物中毒(SFP)。进行这项研究以调查MRSA在肉鸡鸡肉和内脏肉中的流行性和肠毒性。总共5.5%(8/144)的检测样品被mec A阳性/ mec C阴性MRSA污染,在乳房,腿和g的样本中葡萄球菌计数约为10 2集落形成单位(CFU)/ g,冷冻肝样品中的3.3×10 3 CFU / g。大多数MRSA分离株(75%,6/8)带有葡萄球菌肠毒素B(seb)基因。逆转录PCR(RT-PCR)显示,MRSA分离物在温度超过8°C的储存条件下24小时内,在受实验污染的鸡肝中引发SEB产生。当MRSA计数达到7.3×10 3  ±1.2×10 3 CFU / g样品匀浆时,SEB在24°C时最大生成。目前的研究得出的结论是,埃及的主要肉鸡MRSA分离株具有seb基因。为了减轻SEB的可能产生,特别是在肉鸡肝中,应对冷链家禽产品实施最大“冷藏”期限。

更新日期:2020-05-20
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