Object

To investigate N-acetyl-cysteine (NAC) would able to alleviate liver injury and systemic inflammatory response caused by microwave ablation (MWA) in rats.

Materials and methods

Male Sprague-Dawley rats weighing 150–200 g were randomly divided into sham group (only anesthesia and laparotomy except MWA but with intraperitoneal PBS or NAC solution injection according to different situations), control group (intraperitoneal PBS injection for comparation 2 h prior to MWA), and NAC-treated group (intraperitoneal N-acetyl-cysteine (300 mg/kg) injection 2 h prior to MWA). Experimental rats were sacrificed at 4 h following operation in line with the liver injury severity curve. Liver tissue and serum samples were collected for determination of pathology, apoptosis, macrophages contents and protein expression.

Results

The elevated serum level of liver enzymes, Myeloperoxidase (MPO) and inflammatory factors (TNF-α and CXCL1) in MWA-treated rats revealed injurious and pro- inflammatory effect of MVA. Macrophages aggregation was detected in MWA exposure rats similarly. and NAC pre-conditioning mitigate liver damage and hepatocyte apoptosis, besides macrophages accumulation and following inflammatory response in liver tissue.

Conclusion

Our results demonstrated that N-acetyl-cysteine application alleviate macrophages aggregation and inflammatory response in liver suffering microwave ablation, and mitigating liver injury and cell apoptosis.

中文翻译：

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N-乙酰半胱氨酸通过抑制微波消融后巨噬细胞介导的炎症反应减轻肝脏损伤。

目的

研究N-乙酰半胱氨酸（NAC）能够减轻大鼠微波消融（MWA）引起的肝损伤和全身性炎症反应。

材料和方法

将体重150–200 g的雄性Sprague-Dawley大鼠随机分为假手术组（仅麻醉和剖腹手术，MWA除外，但根据不同情况采用腹膜内PBS或NAC溶液注射），对照组（MWA前2 h腹腔注射PBS进行比较） ）和NAC治疗组（在MWA前2小时腹膜内注射N-乙酰半胱氨酸（300 mg / kg））。根据肝损伤严重性曲线，在手术后4小时处死实验大鼠。收集肝组织和血清样品以测定病理学，细胞凋亡，巨噬细胞含量和蛋白质表达。

结果

在MWA处理的大鼠中，肝酶，髓过氧化物酶（MPO）和炎性因子（TNF-α和CXCL1）的血清水平升高，显示出MVA的有害和促炎作用。在MWA暴露大鼠中类似地检测到巨噬细胞聚集。NAC预处理可减轻肝脏损伤和肝细胞凋亡，此外还可以减轻巨噬细胞的积累和肝脏组织中的炎症反应。

结论

我们的结果表明，N-乙酰基半胱氨酸的应用减轻了微波消融肝脏中巨噬细胞的聚集和炎症反应，并减轻了肝脏的损伤和细胞凋亡。