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Serum amyloid P-component/C-reactive proteins in fugu (Takifugu rubripes) egg with binding ability to disease-causing bacteria by carbohydrate-recognition.
Developmental & Comparative Immunology ( IF 2.7 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.dci.2020.103748
Shigeyuki Tsutsui 1 , Shintaro Matsui 1 , Osamu Nakamura 1
Affiliation  

Two galactose-binding proteins were purified from the eggs of Takifugu rubripes by affinity chromatography. These proteins were detected at 26 and 23 kDa under reducing and at 40 and 45 kDa under non-reducing conditions at SDS-PAGE. The peptide sequences from both proteins matched to short-type pentraxin. The 26-kDa lectin was glycosylated, while the other one was not, indicating that these could be glycoforms of pentraxin. Messenger RNA of pentraxin was detected in eggs and embryos at 1-cell stage, was undetectable till blastula, and finally detected again after gastrula, suggesting that the mRNAs in eggs and 1-cell embryos were maternal in origin, and autologous transcription of the gene occurred after blastula. Since they bind to pathogenic bacteria, egg pentraxins may have immunological functions during embryogenesis. This is the first study to show the presence of short-type pentraxin in fish eggs and the diversity of fish egg lectins.

中文翻译:

河豚(Takifugu rubripes)鸡蛋中血清淀粉样蛋白 P 成分/C 反应蛋白通过碳水化合物识别与致病细菌结合。

通过亲和层析从 Takifugu rubripes 的卵中纯化了两种半乳糖结合蛋白。SDS-PAGE 在还原条件下检测到 26 kDa 和 23 kDa 以及在非还原条件下检测到 40 kDa 和 45 kDa 的这些蛋白质。两种蛋白质的肽序列都与短型五聚体相匹配。26-kDa 凝集素被糖基化,而另一种没有,表明这些可能是五聚体的糖型。1细胞期卵子和胚胎中检测到pentraxin的信使RNA,直到囊胚才检测到,最后在原肠胚后再次检测到,表明卵子和1细胞胚胎中的mRNAs是母源性的,基因的自体转录发生在囊胚后。由于它们与病原菌结合,蛋五联蛋白在胚胎发生过程中可能具有免疫功能。
更新日期:2020-05-20
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