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Use of the high-content imaging system equipment to evaluate in vitro infection by Leishmania braziliensis in response to sand fly Nyssomyia neivai saliva.
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.actatropica.2020.105540 Thais Cristina Tirado 1 , Andrey José de Andrade 2 , Magda Clara Vieira da Costa Ribeiro 2 , Fabiano Borges Figueiredo 3
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.actatropica.2020.105540 Thais Cristina Tirado 1 , Andrey José de Andrade 2 , Magda Clara Vieira da Costa Ribeiro 2 , Fabiano Borges Figueiredo 3
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Earlier research has shown that in vivo immunization with sand fly saliva protects the host against infection by parasites of genus Leishmania, and inoculation of saliva along with Leishmania promastigotes favors infection in the host. In this study, High-Content Imaging System was used to demonstrate in vitro that sand fly saliva also promotes infection by these parasites. THP-1 cells were cultured in 96-well microplates and challenged with three strains of Leishmania braziliensis plus four dilutions of Nyssomyia neivai salivary gland extract. High-Content Imaging System equipment (Operetta CLS, Perkin Elmer) was configured to automatically count both cells and parasites inside the microplates and subsequently calculate the Infection Index (II). Results demonstrate that the extract concentration of 1 gland showed greater infection than other dilutions. These findings suggest that sand fly N. neivai saliva has potential for increasing the parasite infection, reinforcing the importance of studying its components. A new method to evaluate Leishmania infection in vitro assays was also presented, broadening this area of study.
中文翻译：
使用高内涵成像系统设备评估巴西利什曼原虫对沙蝇Nyssomyia neivai唾液的体外感染。
较早的研究表明，用沙蝇唾液进行体内免疫可保护宿主免受利什曼原虫属寄生虫的感染，而唾液与利什曼原虫前鞭毛体的接种则有利于宿主感染。在这项研究中，高内涵成像系统用于体外证明沙蝇唾液也促进了这些寄生虫的感染。在96孔微孔板中培养THP-1细胞，并用3株巴西利什曼原虫和4株Nyssomyia neivai唾液腺提取物稀释液攻击。配置高内涵成像系统设备（Operetta CLS，珀金埃尔默公司）以自动计数微孔板内的细胞和寄生虫，然后计算感染指数（II）。结果表明，提取物浓度为1腺比其他稀释度显示出更大的感染力。这些发现表明，沙蝇N. neivai唾液具有增加寄生虫感染的潜力，从而增强了研究其成分的重要性。还提出了一种评估利什曼原虫感染体外测定的新方法，拓宽了这一研究领域。
更新日期：2020-05-20
中文翻译：
使用高内涵成像系统设备评估巴西利什曼原虫对沙蝇Nyssomyia neivai唾液的体外感染。
较早的研究表明，用沙蝇唾液进行体内免疫可保护宿主免受利什曼原虫属寄生虫的感染，而唾液与利什曼原虫前鞭毛体的接种则有利于宿主感染。在这项研究中，高内涵成像系统用于体外证明沙蝇唾液也促进了这些寄生虫的感染。在96孔微孔板中培养THP-1细胞，并用3株巴西利什曼原虫和4株Nyssomyia neivai唾液腺提取物稀释液攻击。配置高内涵成像系统设备（Operetta CLS，珀金埃尔默公司）以自动计数微孔板内的细胞和寄生虫，然后计算感染指数（II）。结果表明，提取物浓度为1腺比其他稀释度显示出更大的感染力。这些发现表明，沙蝇N. neivai唾液具有增加寄生虫感染的潜力，从而增强了研究其成分的重要性。还提出了一种评估利什曼原虫感染体外测定的新方法，拓宽了这一研究领域。
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