OBJECTIVE This study was performed to investigate the effect of hypothermic machine perfusion (HMP) after cold storage (CS) on ovarian transplantation. METHODS Rats aged 8-10 weeks were used as the donors and recipients for allotransplantation. Eighteen donor rats were divided into three groups: the fresh control (n = 6), cold storage (CS; n = 6), and hypothermic machine perfusion (HMP; n = 6) groups. The preservation solution contained Dulbecco's modified Eagle's medium/Ham's F-12 (1:1, v/v), 10% fetal bovine serum, 10 μg/ml insulin, 10 μg/ml transferrin, and 50 mIU/ml follicle-stimulating hormone (FSH). The donor ovaries in the CS and HMP groups were excised and then respectively subjected to 4 h of CS and 2 h of CS combined with 2 h of HMP at 4 °C, and then transplanted beneath the recipient's left renal capsule. At 7 days after transplantation, the ovaries were removed and blood samples were obtained for histological analysis, immunohistochemistry for CD31 and Ki67, and serum anti-Mullerian hormone (AMH) level estimation. RESULTS The HMP group showed significant increases in serum AMH and CD31-positive areas when compared to these values in the CS group (P < 0.05). However, no differences were noted in the total number of follicles or the Ki67-positive areas among the three groups. CONCLUSION Hypothermic machine perfusion after static cold storage is more effective than static CS alone for the short-term preservation of whole ovaries during transport. Whole ovary transplantation with vascular pedicle is our future research direction. Graphical Abstract The black rectangle in the figure shows the place where ligation and disconnection are required, the black dotted line shows the place where vascular forceps are used to clamp, and the black circle shows the place where the cannula is inserted This diagram was made for reviewers to understand more intuitively how my hypothermia mechanical perfusion model was built. Organs obtained in this way can be used for subsequent perfusion and whole ovarian transplantation.

中文翻译：

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静态冷藏后的低温机灌注可改善大鼠卵巢组织移植的卵巢功能。

目的进行本研究以研究冷藏（CS）后低温机器灌注（HMP）对卵巢移植的影响。方法将8-10周龄的大鼠作为同种异体移植的供体和受体。将十八只供体大鼠分为三组：新鲜对照组（n = 6），冷藏（CS； n = 6）和低温体温灌注（HMP； n = 6）组。保存液包含Dulbecco改良的Eagle's培养基/ Ham's F-12（1：1，v / v），10％的胎牛血清，10μg/ ml的胰岛素，10μg/ ml的转铁蛋白和50 mIU / ml的促卵泡激素（FSH）。切开CS和HMP组的供体卵巢，然后分别在4°C下接受4 h CS和2 h CS与2 h HMP结合，然后移植到接受者的左肾囊下方。移植后第7天，摘除卵巢并获得血液样本进行组织学分析，CD31和Ki67的免疫组织化学分析以及血清抗穆勒激素（AMH）水平估计。结果与CS组相比，HMP组的血清AMH和CD31阳性面积显着增加（P <0.05）。但是，三组之间的卵泡总数或Ki67阳性区域总数没有差异。结论静态冷藏后的低温热灌注比单独使用静态CS更能有效保护运输期间的整个卵巢。全卵巢带蒂血管移植是我们未来的研究方向。图形摘要图中的黑色矩形表示需要结扎和断开的位置，黑色虚线显示了使用血管钳夹紧的位置，黑色圆圈显示了插入套管的位置。此图旨在让审核者更直观地了解我的体温过低机械灌流模型是如何构建的。以这种方式获得的器官可用于随后的灌注和整个卵巢移植。