This study was designed to isolate microalgal species which can tolerate severe environmental stresses and can be used for wastewater treatment and biofuel generation. Three different microalgal strains (including Chlorella sorokiniana GEEL-01, Parachlorella kessleri GEEL-02, and Parachlorella kessleri GEEL-03) were isolated from a wastewater treatment plant and Waterwheel Park. The highest growth rate (1.61 OD_680nm) was observed for C. sorokiniana GEEL-01 with highest removal of total nitrogen (1.5 mg L⁻¹ day⁻¹) and total phosphorus (1.82 mg L⁻¹ day⁻¹). The biocomponents of the isolated species ranged from 19.47–29.64%, 39.39–52.51%, and 15.08–22.75% for carbohydrate, protein, and lipid, respectively, based on the dry cell weight (DCW). The function groups in microalgal biocompounds were also confirmed by Fourier-transform infrared radiation (FT-IR) spectra, indicated their ability for biofuel generation. Ultimate and proximate analyses of C. sorokiniana GEEL-01 showed high carbon (54.24% DCW) and volatile content (92.69% DCW), compare to other strains. Moreover, it exhibited a significant accumulation of lipid with an increase in the fractions of saturated fatty acids (64.5%), and the major fatty acids were palmitic acid (11.17%), stearic acid (33.86%), oleic acid (9.84%), and linoleic acid (11.56%). Biodiesel derived from the major fatty acids had optimum engine efficiency properties including iodine value (77.34 g I₂/100 g oil) and cetane number (54.83). Cultivation of C. sorokiniana GEEL-01 can serve as a dual function of biodiesel feedstock generation and nutrients removal.

这项研究旨在分离能够耐受严重环境压力的微藻物种，并将其用于废水处理和生物燃料的生产。从废水处理厂和水车公园分离出了三种不同的微藻菌株（包括小球藻小球藻GEEL-01，凯斯勒小球藻GEEL-02和凯斯小球藻GEEL-03）。马铃薯C. sorokiniana GEEL-01的生长速率最高（1.61 OD _680nm），总氮（1.5 mg L ^-1 day ^-1）和总磷（1.82 mg L ^-1 day ^-1）的去除率最高。）。根据干细胞重量（DCW），分离的物种的碳水化合物，蛋白质和脂质的生物成分分别为19.47–29.64％，39.39–52.51％和15.08–22.75％。微藻生物化合物中的功能基团也通过傅立叶变换红外辐射（FT-IR）光谱进行了确认，表明了它们的生物燃料生成能力。C. sorokiniana的终极和最近分析与其他菌株相比，GEEL-01具有高碳含量（54.24％DCW）和挥发物含量（92.69％DCW）。此外，它显示出大量的脂质积累，其中饱和脂肪酸的比例增加（64.5％），主要脂肪酸为棕榈酸（11.17％），硬脂酸（33.86％），油酸（9.84％）。和亚油酸（11.56％）。生物柴油从主要的脂肪酸衍生的具有最佳效率的发动机性能，包括碘值（77.34克我₂ / 100g的油）和十六烷值（54.83）。C. sorokiniana GEEL-01的培养可以作为生物柴油原料生成和营养去除的双重功能。