当前位置: X-MOL 学术Analyst › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A cost-effective fluorescence biosensor for cocaine based on a "mix-and-detect" strategy.
Analyst ( IF 3.6 ) Pub Date : 2020-05-19 , DOI: 10.1039/d0an00675k
Zhifang Wu 1 , Hui Zhou , Qianjin Han , Xuelian Lin , Dongxue Han , Xun Li
Affiliation  

The efficient detection of illicit drugs such as cocaine continues to be important for the fight against drug trafficking. Herein, we report a one-step method for rapid and specific cocaine detection. The method is based on our finding that small-molecule Thioflavin T (ThT) can act as a fluorescence indicator, which can be bonded with the anti-cocaine aptamer (MNS-4.1) to generate an enhanced fluorescence signal. More interestingly, upon cocaine binding, the intercalated ThT can be replaced, causing a drastic fluorescence reduction. We further optimized the sequence of MNS-4.1 and a new anti-cocaine aptamer (coc.ap2-GC) was obtained. This aptamer showed a higher affinity to both ligands, which increased the ThT binding fluorescence intensity and showed the highest quenching efficiency. Based on the fluorescence change induced by competitive binding, cocaine detection could be accomplished by a “mix-and-detect” strategy within seconds. Such a label-free method exhibits high sensitivity to cocaine with a low detection limit of 250 nM. Moreover, the practical sample analysis (2.5% human urine and saliva) also exhibits good precision and high sensitivity.

中文翻译:

一种基于“混合并检测”策略的经济有效的可卡因荧光生物传感器。

有效打击可卡因等非法药物对于打击贩毒仍然很重要。在本文中,我们报告了一种用于快速和特异性可卡因检测的一步法。该方法基于我们的发现,即小分子硫黄素T(ThT)可以充当荧光指示剂,可以与抗可卡因适体(MNS-4.1)结合以产生增强的荧光信号。更有趣的是,结合可卡因后,嵌入的ThT可以被替换,从而导致荧光急剧降低。我们进一步优化了MNS-4.1的序列,并获得了新的抗可卡因适体(coc.ap2-GC)。该适体对两个配体均显示出更高的亲和力,从而增加了ThT结合荧光强度并显示出最高的猝灭效率。基于竞争性结合诱导的荧光变化,可卡因检测可以通过“混合检测”策略在几秒钟内完成。这种无标记方法显示出对可卡因的高灵敏度,检测限低至250 nM。此外,实用的样品分析(2.5%的人类尿液和唾液)也具有良好的精密度和高灵敏度。
更新日期:2020-06-29
down
wechat
bug