Embryos from the marine medaka fish Oryzias javanicus were treated with eight concentrations of benzo[a]pyrene (BaP) (0.001, 0.01, 0.1, 1, 2, 5, 10, or 20 μg L^-–1) after they had been fertilized. Significant mortality and hatching delays were detected in embryos that had been exposed to 10 and 20 μg L⁻¹ BaP for 4 weeks. The mortality rate after hatching was higher in the medaka that had been previously exposed to > 2 μg L⁻¹ BaP. Significant elevations in intracellular reactive oxygen species and malondialdehyde contents were measured and the mRNA expressions of the antioxidant defense system genes (gst, sod, cat, and gpx) increased in the embryos exposed to 10 and 20 μg L⁻¹ BaP for 1 week. The hsp70, ahr, and cyp1a transcriptional responses were also significantly upregulated in the exposed groups after 1 week. The alterations to the in vivo parameters and molecular components suggested that waterborne BaP had a toxic effect on marine medaka embryos. Finally, fin defects, spinal curvature, and cardiac edema were highly induced when the embryos were exposed to > 5 μg L⁻¹ BaP.

从海洋鱼青鳉胚胎爪哇青鳉用8个浓度苯并[处理一个]芘（BAP）（0.001,0.01，0.1％，1，2，5，10，或20微克大号^--1），他们已受精后。在暴露于10和20μgL ^-1 BaP 4周的胚胎中，检测到了显着的死亡率和孵化延迟。在之前已暴露于> 2μgL ^-1 BaP的青hat中，孵化后的死亡率较高。测量了细胞内活性氧和丙二醛含量的显着升高，以及抗氧化防御系统基因（gst，sod，cat和gpx）在暴露于10和20μgL ^-1 BaP 1周的胚胎中增加。该HSP70，AHR和CYP1A转录反应也显著1周后上调暴露组。到的改变在体内参数和分子成分建议水性的BaP对海洋青鳉胚胎毒性作用。最后，当胚胎暴露于> 5μgL ^-1 BaP时，高度诱发了鳍缺损，脊柱弯曲和心脏水肿。