Abstract Bilirubin oxidases (BODs), which catalyze the oxidation of bilirubin to biliverdin, are multi-copper oxidases that can be used in clinical diagnoses. Given their different reported enzymatic characterizations from laccases, BODs have gained considerable attention for their application in healthcare and environment management. In this study, a BOD gene from Myrothecium verrucaria was chemically synthesized and optimized to heterogeneous expression in Arabidopsis thaliana. The optimized DNA sequence showed 80.1% similarity to the original sequences. The gene was successfully transferred into A. thaliana, and the crude extracts of transgenic plants showed higher BOD activity compared with that of untransformed plants. BOD expressed in transgenic A. thaliana also exhibited an activity on the typical substrate (ABTS) of laccase and was able to decolorize the textile dyes crystal violet and malachite green. The results indicated that the M. verrucaria BOD gene could be transgenically exploited in plants for dye decolorization.

中文翻译：

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拟南芥中疣状漆斑菌胆红素氧化酶基因的异源表达与表征

摘要 胆红素氧化酶（BODs）是一种多铜氧化酶，可用于临床诊断，催化胆红素氧化为胆绿素。鉴于它们与漆酶不同的酶学特征，BOD 因其在医疗保健和环境管理中的应用而获得了相当多的关注。在这项研究中，来自疣孢漆霉的 BOD 基因被化学合成并优化为在拟南芥中的异质表达。优化后的 DNA 序列与原始序列的相似度为 80.1%。该基因成功转入拟南芥，转基因植物的粗提物与未转化植物相比显示出更高的BOD活性。BOD 在转基因 A. 拟南芥还对漆酶的典型底物 (ABTS) 表现出活性，并且能够使纺织染料结晶紫和孔雀石绿脱色。结果表明M.verrucaria BOD基因可以在植物中进行转基因开发用于染料脱色。