ABSTRACT

Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust contains extracellular vesicles (EVs), which are associated with pulmonary inflammation. However, it has not been reported whether indoor dust EVs affect the cancer lung metastasis. In this study, we isolated indoor dust EVs and investigated their roles in cancer lung metastasis. Upon intranasal administration, indoor dust EVs enhanced mouse melanoma lung metastasis in a dose-dependent manner in mice. Pre-treatment or co-treatment of indoor dust EVs significantly promoted melanoma lung metastasis, whereas post-treatment of the EVs did not. In addition, the lung lysates from indoor dust EV-treated mice significantly increased tumour cell migration in vitro. We observed that tumour necrosis factor-α played important roles in indoor dust EV-mediated promotion of tumour cell migration in vitro and cancer lung metastasis in vivo. Furthermore, Pseudomonas EVs, the main components of indoor dust EVs, and indoor dust EVs showed comparable effects in promoting tumour cell migration in vitro and cancer lung metastasis in vivo. Taken together, our results suggest that indoor dust EVs, at least partly contributed by Pseudomonas EVs, are potential promoting agents of cancer lung metastasis.

摘要

室内污染物是公共卫生的重要问题。在室内污染物中，室内灰尘中含有与肺部炎症相关的细胞外囊泡（EVs）。但是，尚未报道室内灰尘电动车是否会影响癌症的肺转移。在这项研究中，我们隔离了室内粉尘电动汽车，并研究了它们在癌症肺转移中的作用。鼻内给药后，室内粉尘电动车以剂量依赖性方式增强小鼠黑色素瘤的肺转移。室内粉尘电动车的预处理或共同处理可显着促进黑色素瘤肺转移，而电动车的后处理则不能。此外，室内粉尘电动车治疗小鼠的肺裂解液显着增加了体外肿瘤细胞的迁移。我们观察到肿瘤坏死因子-α在室内粉尘EV介导的体外肿瘤细胞迁移和体内癌肺转移的促进中起着重要作用。此外，假单胞菌电动汽车，室内粉尘电动汽车的主要成分和室内粉尘电动汽车在促进体外肿瘤细胞迁移和体内癌性肺转移方面显示出可比的效果。两者合计，我们的结果表明，室内粉尘电动汽车（至少部分由假单胞菌电动汽车促成）是潜在的癌症肺癌转移促进剂。