ABSTRACT

Introduction

The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research.

Areas covered

We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses.

Expert commentary

The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.

中文翻译：

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蛋白质生物标志物多重分析的分析技术。

摘要

介绍

在当前向个性化医学的转变中，生物标志物对于药物开发和临床诊断的重要性比以往任何时候都更加重要。生物标志物已成为中心标志，既可以作为辅助标志物来支持药物开发和患者选择，也可以作为指标来检测最早的疾病扰动，从而最大程度地减少治疗干预甚至使疾病逆转。鉴于蛋白质生物标记物在生化途径中的重要作用，因此特别受到关注。因此，对于一种生物医学研究而言，在一种测定法中分析多种蛋白质生物标志物的能力非常受关注。

覆盖区域

我们在这里回顾了多种方法，这些方法适用于以多重格式对蛋白质生物标志物进行强大，高通量，标准化和可负担的分析。我们描述了免疫测定法的创新发展，临床实验室方法的领先者和质谱法，这些方法越来越多地用于蛋白质生物标志物分析。此外，讨论了新兴技术，这些技术具有潜在改善的蛋白质捕获，分离和检测能力，将进一步促进多重分析。

专家评论

临床应用的多重蛋白生物标志物测定方法的发展至关重要，因为与单个生物标志物相比，多蛋白标志物可提供有关生物系统的更全面的信息，从而提高了对疾病机理，诊断和个性化药物作用的认识。