Xanthophyllomyces dendrorhous is a promising source of natural astaxanthin due to its ability to accumulate high amounts of astaxanthin. This study showed that 6‐benzylaminopurine (6‐BAP) is an effective substrate that enhances cell biomass and astaxanthin accumulation in X. dendrorhous. In the current study, the biomass and astaxanthin content in X. dendrorhous were determined to be improved by 21.98% and 24.20%, respectively, induced by 6‐BAP treatments. To further understand the metabolic responses of X. dendrorhous to 6‐BAP, time‐course metabolomics and gene expression levels of X. dendrorhous cultures with and without 6‐BAP feeding were investigated. Metabolome analysis revealed that 6‐BAP facilitated glucose consumption, promoted the glycolysis, suppressed the TCA cycle, drove carbon flux of acetyl‐CoA into fatty acid and mevalonate biosynthesis, and finally facilitated the formation of astaxanthin. ROS analysis suggested that the antioxidant mechanism in X. dendrorhous can be induced by 6‐BAP. Additionally, the process of 6‐BAP significantly upregulated the expression of six key genes involved in pathways related to astaxanthin biosynthesis. This research demonstrates the metabolomic mechanism of phytohormone stimulation of astaxanthin production iNn X. dendrorhous and presents a new strategy to improve astaxanthin production to prevent the dilemma of choosing between accumulation of astaxanthin and cell biomass.

中文翻译：

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通过植物激素增强树突状黄单胞菌中虾青素的积累：代谢组学和基因表达谱。

由于其能够积聚大量虾青素的能力，黄果树种是天然虾青素的有前途的来源。这项研究表明，6-苄氨基嘌呤（6-BAP）是一种有效的底物，可增强X. dendrorhous中细胞生物量和虾青素的积累。在当前的研究中，确定了6-BAP处理诱导的牙形木中生物量和虾青素含量分别提高了21.98％和24.20％。为了进一步了解树状支原体对6-BAP的代谢反应，时程代谢组学和树状支原体的基因表达水平研究了有和没有6-BAP喂养的培养物。代谢组学分析表明，6-BAP促进葡萄糖消耗，促进糖酵解，抑制TCA循环，推动乙酰辅酶A的碳通量进入脂肪酸和甲羟戊酸酯的生物合成，并最终促进虾青素的形成。ROS分析表明，6-BAP可以诱导树突触杆菌的抗氧化机理。此外，6-BAP的过程显着上调了与虾青素生物合成相关途径中涉及的六个关键基因的表达。这项研究证明了植物激素刺激虾青素生产iNn X. dendrorhous的代谢组学机理。 提出了提高虾青素产量的新策略，以防止虾青素积累与细胞生物量之间的选择困境。