Pigment epithelium‐derived factor (PEDF) is a multifunctional protein which was initially described in the retina, although it is also present in other tissues. It functions as an antioxidant agent promoting neuronal survival. Recently, a PEDF receptor has shown an elevated binding affinity for PEDF. There are no relevant data regarding the distribution of both proteins in the brain, therefore the main goal of this work was to investigate the spatiotemporal presence of PEDF and PEDFR in the adult mouse brain, and to determine the PEDF blood level in mouse and human. The localization of both proteins was analyzed by different experimental methods such as immunohistochemistry, western‐blotting, and also by enzyme‐linked immunosorbent assay. Differential expression was found in some telencephalic structures and positive signals for both proteins were detected in the cerebellum. The magnitude of the PEDFR labeling pattern was higher than PEDF and included some cortical and subventricular areas. Age‐dependent changes in intensity of both protein immunoreactions were found in the cortical and hippocampal areas with greater reactivity between 4 and 8 months of age, whilst others, like the subventricular zones, these differences were more evident for PEDFR. Although ubiquitous presence was not found in the brain for these two proteins, their relevant functions must not be underestimated. It has been described that PEDF plays an important role in neuroprotection and data provided in the present work represents the first extensive study to understand the relevance of these two proteins in specific brain areas.

中文翻译：

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成年小鼠大脑中的色素上皮衍生因子 (PEDF) 和 PEDF 受体：差异空间/时间定位模式。

色素上皮衍生因子 (PEDF) 是一种多功能蛋白质，最初在视网膜中被描述，尽管它也存在于其他组织中。它作为一种抗氧化剂，促进神经元存活。最近，PEDF 受体显示出对 PEDF 的结合亲和力升高。没有关于这两种蛋白质在大脑中分布的相关数据，因此这项工作的主要目标是调查成年小鼠大脑中 PEDF 和 PEDFR 的时空存在，并确定小鼠和人类的 PEDF 血液水平。两种蛋白质的定位通过不同的实验方法进行分析，如免疫组织化学、蛋白质印迹法以及酶联免疫吸附测定。在一些端脑结构中发现了差异表达，并且在小脑中检测到两种蛋白质的阳性信号。PEDFR 标记模式的幅度高于 PEDF，包括一些皮质和脑室下区域。在 4 至 8 个月大的皮质和海马区域发现两种蛋白质免疫反应强度的年龄依赖性变化，而其他区域，如脑室下区，PEDFR 的这些差异更为明显。虽然在大脑中没有发现这两种蛋白质无处不在，但不能低估它们的相关功能。