Asiatic citrus psylla, Diaphorina citri, Kuwayama (Hemiptera: Liviidae) is an economic pest of citrus groves and a vector of the bacterium, Candidatus Liberibacter spp., one of the causative agents of citrus greening. In order to estimate the infectivity of six different isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea, fungal bioassay was performed on the adults of D. citri. Adults of D. citri were treated individually with 1 × 105, 1 × 106, 1 × 107, 1 × 108, 2 × 108 spores/mL fungal concentrations by the immersion method. Subsequent to fungal bioassay, treated D. citri were used to determine the levels of esterase and glutathione S‐transferases (GST) enzymes over a period of 3–7 days. The mortality results suggest that I. fumosorosea isolates (If‐02) caused 82.2% mortality on the seventh day of treatment. However, B. bassiana isolate (Bb‐08) with lowest LC50 (1.4 × 107 spores/mL) proved to be highest potential isolate against D. citri. Biochemical determination of esterase and GST activity assay showed significant differences in activities after infection of fungi. Significantly high activity of esterase was observed by Bb‐01 (27.0 unit per mg protein) on the seventh day, while Ma‐11.1 and If‐2.3 (16.9 and 36.3 unit per mg protein) on the third day post treatment. However, maximum GST's activity was showen by isolates Bb‐08,Ma‐M2 and If‐2.3 (37.6, 1.40 and 10.9 unit per mg protein) on the third day. The current investigation will help to explore the relations between the insect defense system and entomopathogenic fungi. Moreover, the determination of enzymatic activities will be useful for selecting the most pathogenic isolates.

中文翻译：

---

昆虫病原真菌对柑桔木虱（半翅目：鳢科）的生化分析及致病性

亚洲柑橘木虱，Diaphorina citri，Kuwayama（半翅目：Liviidae）是柑橘园的一种经济害虫，也是柑橘绿化病原之一的细菌念珠菌属（Candidatus Liberibacter spp.）的载体。为了估计球孢白僵菌、绿僵菌和烟叶螟的六种不同分离株的传染性，对柑橘木虱的成虫进行了真菌生物测定。D. citri 的成虫通过浸泡方法用 1 × 105、1 × 106、1 × 107、1 × 108、2 × 108 孢子/mL 真菌浓度单独处理。在真菌生物测定之后，使用处理过的柑橘木虱在 3-7 天的时间内测定酯酶和谷胱甘肽 S 转移酶 (GST) 酶的水平。死亡率结果表明，I. fumosorosea 分离株 (If-02) 在治疗的第 7 天导致 82.2% 的死亡率。然而，B. 具有最低 LC50（1.4 × 107 孢子/mL）的球孢子菌分离物 (Bb-08) 被证明是针对 D. citri 的最高分离物。酯酶生化测定和GST活性测定显示真菌感染后活性存在显着差异。在第 7 天观察到 Bb-01（每毫克蛋白质 27.0 单位）的酯酶活性显着高，而在治疗后第三天观察到 Ma-11.1 和 If-2.3（每毫克蛋白质 16.9 和 36.3 单位）。然而，分离株 Bb-08、Ma-M2 和 If-2.3（每毫克蛋白质 37.6、1.40 和 10.9 单位）在第三天显示出最大的 GST 活性。目前的调查将有助于探索昆虫防御系统与昆虫病原真菌之间的关系。此外，酶活性的测定将有助于选择最具致病性的分离株。