Generation of Footprint-Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector.,Molecular Reproduction and Development

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Generation of Footprint-Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-05-19 , DOI: 10.1002/mrd.23349
Masaya Tsukamoto ₁ , Kazuto Kimura ₁ , Miyuu Tanaka ₂ , Mitsuru Kuwamura ₂ , Manami Ohtaka ₃ , Mahito Nakanishi _{3,

4} , Kikuya Sugiura ₁ , Shingo Hatoya ₁

Affiliation

Using auto‐erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT‐PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.

中文翻译：

使用仙台病毒载体，从外周血单核细胞中产生无足迹的犬诱导多能干细胞。

使用自动擦除的仙台病毒载体，我们生成了ciPSC品系。经过几代之后，通过RT-PCR在ciPSC中不存在病毒。来自犬PBMC的ciPSC具有多能状态，体外分化了所有三个细菌层，并且具有正常的78 XX核型。这些结果证明，PBMC是从伴侣犬和患者犬产生ciPSC系的良好细胞来源之一。

更新日期：2020-06-28

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