当前位置: X-MOL 学术J. Cell. Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Rapid human-derived iPSC osteogenesis combined with three-dimensionally printed Ti6Al4V scaffolds for the repair of bone defects.
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-18 , DOI: 10.1002/jcp.29788
Lingjia Yu 1 , Yong Yang 1 , Bin Zhang 2 , Xiaofeng Bai 2 , Qi Fei 1 , Lei Zhang 2
Affiliation  

Human‐induced pluripotent stem cells (iPSCs) are an alternative source of mesenchymal stem cells used for bone regeneration. However, the current osteogenically induced methods for iPSCs are slow and complex. We have used retinoic acid (RA) to induce osteogenic iPSCs within 10 days and assess whether a rapid differentiation could improve the osteogenic potential of the three‐dimensionally printed Ti6Al4V (3DTi) scaffolds. First, the osteogenic differentiation of iPSCs was induced with RA, and the osteogenic potential of iPSCs was evaluated using standard assays. In addition, a 5‐mm mandibular bone defect was generated in rats and was repaired with 3DTi scaffolds that were seeded with iPSC‐induced osteoblasts. The capacity of seeded scaffolds for the enhancement of bone regeneration in vivo was assessed. Finally, we tested the potential mechanisms of RA‐dependent iPSC bone induction and its effect on the Wnt/β‐catenin pathway. The results showed that iPSCs could form osteocytes within 10 days. Animal experiments confirmed that rapid osteo‐induced iPSCs could enhance the bone regeneration and osteointegration capacity of the 3DTi scaffolds. Mechanistically, RA could activate the AKT/GSK3β/β‐catenin pathway during the process of iPSCs osteogenesis. The rapid osteoinduction of iPSCs combined with 3DTi scaffolds is a safe, effective, and reproducible method for repairing mandibular bone defects.

中文翻译:

快速人类衍生的iPSC成骨与三维打印的Ti6Al4V支架相结合,可修复骨缺损。

人诱导多能干细胞(iPSC)是用于骨骼再生的间充质干细胞的替代来源。然而,当前的成骨诱导的iPSC方法缓慢且复杂。我们已经使用视黄酸(RA)在10天内诱导成骨性iPSC,并评估了快速分化是否可以改善三维印刷的Ti6Al4V(3DTi)支架的成骨潜能。首先,用RA诱导iPSC的成骨分化,并使用标准测定法评估iPSC的成骨潜力。此外,在大鼠中产生了一个5毫米的下颌骨缺损,并用植入iPSC诱导的成骨细胞的3DTi支架进行了修复。评估了植入的支架在体内增强骨再生的能力。最后,我们测试了RA依赖性iPSC骨诱导的潜在机制及其对Wnt /β-catenin途径的影响。结果表明,iPSCs可以在10天内形成骨细胞。动物实验证实,快速的骨诱导性iPSC可以增强3DTi支架的骨再生和骨整合能力。从机理上讲,RA可以在iPSC的成骨过程中激活AKT /GSK3β/β-catenin途径。iPSC与3DTi支架的快速骨诱导是修复下颌骨缺损的安全,有效和可重复的方法。动物实验证实,快速的骨诱导性iPSC可以增强3DTi支架的骨再生和骨整合能力。从机理上讲,RA可以在iPSC的成骨过程中激活AKT /GSK3β/β-catenin途径。iPSC与3DTi支架的快速骨诱导是修复下颌骨缺损的安全,有效和可重复的方法。动物实验证实,快速的骨诱导性iPSC可以增强3DTi支架的骨再生和骨整合能力。从机制上讲,RA可以在iPSC的成骨过程中激活AKT /GSK3β/β-catenin途径。iPSC与3DTi支架的快速骨诱导是修复下颌骨缺损的安全,有效和可重复的方法。
更新日期:2020-05-18
down
wechat
bug