The Asian citrus psyllid, Diaphorina citri is the principal vector of huanglongbing, which transmits 'Candidatus Liberibacter asiaticus'. Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and development. The specific functions of this enzyme in D. citri have not been determined. In this study, three trehalase genes (DcTre1-1, DcTre1-2, and DcTre2) were identified based on the D. citri genome database. Bioinformatic analysis showed that DcTre1-1 and DcTre1-2 are related to soluble trehalase, whereas DcTre2 is associated with membrane-bound trehalase. Spatiotemporal expression analysis indicated that DcTre1-1 and DcTre1-2 had the highest expression levels in the head and wing, respectively, and DcTre2 had high expression levels in the fat body. Furthermore, DcTre1-1 and DcTre1-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone Ⅲ, but DcTre2 was unaffected. The expression levels of DcTre1-1, DcTre1-2, and DcTre2 were significantly upregulated, which resulted in high mortality after treatment with validamycin. Trehalase activities and glucose contents were downregulated, but the trehalose content increased after treatment with validamycin. In addition, the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with validamycin. Furthermore, silencing of DcTre1-1, DcTre1-2, and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of D. citri. These results indicate that D. citri trehalase plays an essential role in regulating chitin metabolism and provides a new target for controlling of D. citri. This article is protected by copyright. All rights reserved.

中文翻译：

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抑制海藻糖酶影响柑橘木虱（半翅目：木虱科）中的海藻糖和几丁质代谢途径

亚洲柑橘木虱，Diaphorina citri 是黄龙病的主要媒介，它传播'Candidatus Liberibacter asiaticus'。海藻糖酶是参与海藻糖水解的关键酶，在昆虫生长发育中起着重要作用。这种酶在 D. citri 中的具体功能尚未确定。在本研究中，基于柑橘果蝇基因组数据库确定了三个海藻糖酶基因（DcTre1-1、DcTre1-2 和 DcTre2）。生物信息学分析表明，DcTre1-1 和 DcTre1-2 与可溶性海藻糖酶有关，而 DcTre2 与膜结合海藻糖酶有关。时空表达分析表明，DcTre1-1和DcTre1-2分别在头部和翅膀中表达水平最高，DcTre2在脂肪体中表达水平最高。此外，DcTre1-1和DcTre1-2的表达水平受到20-羟基蜕皮激素和保幼激素Ⅲ的诱导，但DcTre2不受影响。DcTre1-1、DcTre1-2和DcTre2的表达水平显着上调，导致有效霉素治疗后死亡率高。海藻糖酶活性和葡萄糖含量下调，但海藻糖含量在用有效霉素处理​​后增加。此外，几丁质代谢相关基因的表达水平在用验证霉素处理后24和48小时显着降低。此外，DcTre1-1、DcTre1-2 和 DcTre2 的沉默降低了几丁质代谢相关基因的表达水平，并导致柑橘 DcTre 的畸形表型。这些结果表明 D. citri 海藻糖酶在调节几丁质代谢中起重要作用，为控制柑橘果蝇提供了新的靶点。本文受版权保护。版权所有。