Endophytes live asymptomatically within many cool‐season grasses for at least a portion of their life cycle. Although the leaf endophyte effects on the growth and stress resistance of host grasses have been relatively well studied, less attention has been focused on how this relationship may impact soil microbial communities, especially arbuscular mycorrhizal fungi (AMF). We defined endophyte‐infected and endophyte‐free Leymus chinensis as the host grass and neighbouring Stipa krylovii, which was not infected by endophytes, as the non‐host grass. Our objective was to examine the effect of endophyte infection on the colonization rate and composition of AMF communities in both the rhizosphere and bulk soils of their host (L. chinensis) and a neighbouring non‐host (S. krylovii) by a consecutive 2‐year survey. We described AMF communities using high‐throughput DNA sequencing, quantified the proportion of root length colonized by AMF using microscopy, and estimated AMF biomass using a lipid biomarker. Structural equation modelling (SEM) was applied to disentangle the direct and indirect effects (mediated by multiple environmental factors) of an endophyte on AMF. We found that endophyte infection significantly increased colonization rates and rhizosphere soil biomass of AMF but had no significant effect on the bulk soil around the host grass. The endophyte increased the diversity of the rhizosphere soil AMF community by decreasing the abundance of the dominant species Glomus while increasing the abundance of some unidentified AMF. SEM showed that the endophyte effects on AMF biomass in rhizosphere soil were primarily mediated by soil total carbon, total nitrogen and pH. With respect to the non‐host grass, the endophyte infection of host grass did not change the colonization rates or AMF biomass in either the rhizosphere or bulk soil but did cause a shift in the rhizosphere soil AMF community composition, resulting in a decrease in the relative abundance of Septoglomus and an increase in the relative abundance of some unidentified AMF.

中文翻译：

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内生细菌感染影响寄主与非寄主草相反的根际土壤中的丛枝菌根真菌群落

内生菌至少在生命周期的一部分内无症状地生活在许多凉季草中。尽管叶片内生菌对寄主草的生长和抗逆性的影响已得到相对较好的研究，但人们很少关注这种关系如何影响土壤微生物群落，尤其是丛枝菌根真菌（AMF）。我们将受内生菌感染和无内生菌的羊草定义为寄主草，未受内生菌感染的邻近的针茅克雷洛夫作为非寄主草。我们的目的是研究内生菌感染对其寄主（中华绒螯蟹）根际和土壤中定殖率和AMF群落组成的影响。），以及连续两年进行调查的相邻非寄主（S. krylovii）。我们使用高通量DNA测序技术描述了AMF群落，使用显微镜定量了AMF定殖的根长比例，并使用脂质生物标记物估计了AMF生物量。应用结构方程模型（SEM）来分析内生菌对AMF的直接和间接影响（由多种环境因素介导）。我们发现内生菌感染显着提高了AMF的定殖率和根际土壤生物量，但对寄主草周围的大量土壤没有显着影响。内生菌通过降低优势种Glomus的丰度，增加了根际土壤AMF群落的多样性。同时增加了一些身份不明的AMF的数量。SEM表明，内生菌对根际土壤AMF生物量的影响主要是由土壤总碳，总氮和pH介导的。就非寄主草而言，寄主草的内生菌感染并没有改变根际或块状土壤中的定殖率或AMF生物量，但确实引起了根际土壤AMF群落组成的变化，从而导致根际土壤AMF群落的减少。的相对丰度Septoglomus，并增加了一些身份不明的AMF的相对丰度。