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The prevalence of Rickettsia felis DNA in fleas collected from cats and dogs in the UK.
Veterinary Parasitology ( IF 2.6 ) Pub Date : 2020-05-19 , DOI: 10.1016/j.vetpar.2020.109143
Swaid Abdullah 1 , Phillipa Lait 2 , Chris Helps 2 , Hannah Newbury 3 , Richard Wall 4
Affiliation  

In a large-scale survey in the UK, recruited veterinary practices were asked to inspect client-ownedcats and dogs, selected at random between April and June 2018, following a standardised flea inspection protocol. A total of 326 veterinary practices participated and 812 cats and 662 dogs were examined during the 3-month period. Fleas were collected, identified to species level and fleas of the same species collected from a single animal were pooled together and treated as a single sample. A total of 470 pooled flea samples were screened by PCR and DNA sequence analysis for a subset of Rickettsia species including R. felis and R. typhi. On analysis, 27 (5.7%) of the pooled flea samples were positive for R. felis DNA; these were predominantly in the cat flea, Ctenocephalides felis, but one dog flea, Ctenocephalides canis was also positive for this pathogen.



中文翻译:

在英国,从猫和狗采集的跳蚤中有立克次体猫的DNA流行。

在英国进行的一项大规模调查中,按照标准化的跳蚤检查规程,要求招募的兽医部门对客户拥有的猫狗进行检查,这些猫狗是在2018年4月至6月之间随机选择的。在三个月的时间内,总共参加了326次兽医检查,检查了812只猫和662只狗。收集蚤,鉴定到物种水平,将从单个动物收集的相同物种的蚤汇集在一起​​,并作为单个样品处理。共有470个汇集蚤样品通过PCR和DNA序列分析的子集筛选立克次氏体的物种,包括R.蚤R.伤寒沙门氏菌。经分析,合并的跳蚤样品中有27株(5.7%)的R. felis呈阳性脱氧核糖核酸; 这些主要在猫跳蚤Ctenocephalides felis中出现,但其中一只狗跳蚤Ctenocephalides canis也对该病原体呈阳性反应。

更新日期:2020-05-19
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