The classical swine (CS) H1N1 swine influenza virus (SIVs) emerged in humans as a reassortant virus that caused the H1N1 influenza virus pandemic in 2009, and the European avian-like (EA) H1N1 SIVs has caused several human infections in European and Asian countries. Development of the influenza vaccines that could provide effective protective efficacy against SIVs remains a challenge. In this study, the bivalent reassortant inactivated vaccine comprised of SH1/PR8 and G11/PR8 arboring the hemagglutinin (HA) and neuraminidase (NA) genes from prevalent CS and EA H1N1 SIVs and six internal genes from the A/Puerto Rico/8/34(PR8) virus was developed. The protective efficacy of this bivalent vaccine was evaluated in mice challenged with the lethal doses of CS and EA H1N1 SIVs. The result showed that univalent inactivated vaccine elicited high-level antibody against homologous H1N1 viruses while cross-reactive antibody responses to heterologous H1N1 viruses were not fully effective. In a mouse model, the bivalent inactivated vaccine conferred complete protection against lethal challenge doses of EA SH1 virus or CS G11 virus, whereas the univalent inactivated vaccine only produced insufficient protection against heterologous SIVs. In conclusion, our data demonstrated that the reassortant bivalent inactivated vaccine comprised of SH1/PR8 and G11/PR8 could provide effective protection against the prevalent EA and CS H1N1 subtype SIVs in mice.

2009年，经典猪（CS）H1N1猪流感病毒（SIV）作为重组病毒在人类中引起H1N1流感病毒大流行，欧洲禽类（EA）H1N1 SIVs在欧洲和亚洲引起了数次人类感染国家。可以提供针对SIV的有效保护功效的流感疫苗的开发仍然是一个挑战。在这项研究中，由SH1 / PR8和G11 / PR8组成的二价重配灭活疫苗含有来自流行的CS和EA H1N1 SIV的血凝素（HA）和神经氨酸酶（NA）基因以及来自A / Puerto Rico / 8 /的六个内部基因开发了34（PR8）病毒。在用致死剂量的CS和EA H1N1 SIV攻击的小鼠中评估了这种二价疫苗的保护功效。结果表明，单价灭活疫苗可引发针对同源H1N1病毒的高水平抗体，而对异源H1N1病毒的交叉反应抗体反应则不能完全有效。在小鼠模型中，二价灭活疫苗赋予了对致命剂量的EA SH1病毒或CS G11病毒的完全保护，而单价灭活疫苗仅针对异源SIV产生了不足的保护。总之，我们的数据表明，由SH1 / PR8和G11 / PR8组成的重配双价灭活疫苗可以有效抵抗小鼠中常见的EA和CS H1N1亚型SIV。二价灭活疫苗可提供针对致命攻击剂量的EA SH1病毒或CS G11病毒的完全保护，而单价灭活疫苗仅能产生对异源SIV的足够保护。总之，我们的数据表明，由SH1 / PR8和G11 / PR8组成的重配双价灭活疫苗可以有效抵抗小鼠中常见的EA和CS H1N1亚型SIV。二价灭活疫苗可提供针对致命攻击剂量的EA SH1病毒或CS G11病毒的完全保护，而单价灭活疫苗仅能产生对异源SIV的足够保护。总之，我们的数据表明，由SH1 / PR8和G11 / PR8组成的重配双价灭活疫苗可以有效抵抗小鼠中常见的EA和CS H1N1亚型SIV。