Two isolates, one recovered from a carrot and another one from urban wastewater, were characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates clustered together, and were most closely related to Aliarcobacter lanthieri. Multilocus phylogenetic analysis (MLPA) using the concatenated sequences of five housekeeping genes (atpA, gyrA, gyrB, hsp60 and rpoB) suggested that these isolates formed a distinct phylogenetic lineage among the genera derived from the former genus Arcobacter. Whole-genome sequence, in silico DNA-DNA hybridization (isDDH) and the average nucleotide identity (ANI) value between the genome of strain F199^T and those of related species confirmed that these isolates represent a novel species. These strains can be differentiated from its phylogenetically closest species A. lanthieri by its inability to growth on 1% glycine and by their enzyme activity of esterase lipase (C8) and acid phosphatase. Our results, by the application of a polyphasic analysis, confirmed that these two isolates represent a novel species of the genus Aliarcobacter, for which the name Aliarcobacter vitoriensis sp. nov. is proposed. The type strain is F199^T (=CECT 9230^T=LMG 30050^T).

使用多相法对两种分离株进行了表征，一种从胡萝卜中回收，另一种从城市废水中回收。基于16S rRNA基因序列的系统发育分析表明，这两个分离株聚集在一起，并且与兰科拟杆菌属（Aararcobacter lanthieri）关系最密切。使用五个管家基因（atpA，gyrA，gyrB，hsp60和rpoB）的串联序列进行的多基因座系统发育分析（MLPA）表明，这些分离株在原属杆状杆菌属的属中形成了独特的系统发育谱系。全基因组序列，在计算机芯片上的DNA-DNA杂交（是D199）和F199 ^T菌株基因组与相关物种的基因组之间的平均核苷酸同一性（ANI）值证实了这些分离物代表了一个新物种。这些菌株可以通过在1％甘氨酸上无法生长以及其酯酶脂肪酶（C8）和酸性磷酸酶的酶活性而与系统发育最接近的物种A. lanthieri区别开来。我们的结果，通过多相分析的应用，证实这两个分离株代表Aliarcobacter属的一个新物种，其名称为Aliarcobacter vitoriensis sp。十一月 被提议。类型应变为F199 ^T（= CECT 9230 ^T = LMG 30050 ^T）。