Introduction

Vascular endothelial cell disorders are closely related to cardiovascular disease (CVD) and pulmonary diseases. Abnormal lipid metabolism in the endothelium leads to changes in cell signalling, and the expression of genes related to immunity and inflammation. It is therefore important to investigate the pathophysiology of vascular endothelial disorders in terms of lipid metabolism, using a disease model of endothelium.

Methods

Human induced pluripotent stem cell-derived endothelial cells (iECs) were cultured on a matrigel to form an iEC network. Lipids in the iEC network were investigated by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass spectrometry (IMS) analysis. Ion fragments obtained by mass spectrometry were analysed using an infusion method, involving precursor ion scanning with fragment ion.

Results

The MALDI TOF IMS analysis revealed co-localized intensity of peaks at m/z 592.1 and 593.1 in the iEC network. Tandem mass spectrometry (MS/MS) analysis by MALDI-imaging, in conjunction with precursor ion scanning using an infusion method with lipid extracts, identified that these precursor ions were lysophosphatidylcholine (LPC) (22:5) and its isotype.

Conclusion

The MALDI-imaging analysis showed that LPC (22:5) was abundant in an iEC network. As an in vitro test model for disease and potential therapy, present analysis methods using MALDI-imaging combined with, for example, mesenchymal stem cells (MSC) to a disease derived iEC network may be useful in revealing the changes in the amount and distribution of lipids under various stimuli.

中文翻译：

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诱导多能干细胞衍生的内皮细胞网络中溶血磷脂酰胆碱的成像。

介绍

血管内皮细胞疾病与心血管疾病（CVD）和肺部疾病密切相关。内皮中异常的脂质代谢导致细胞信号传导的变化，以及与免疫和炎症相关的基因的表达。因此，使用内皮疾病模型研究血管内皮疾病在脂质代谢方面的病理生理学是很重要的。

方法

在基质胶上培养人诱导多能干细胞衍生的内皮细胞 (iEC) 以形成 iEC 网络。通过基质辅助激光解吸/电离 (MALDI) 飞行时间 (TOF) 成像质谱 (IMS) 分析研究 iEC 网络中的脂质。使用注入法分析通过质谱法获得的离子碎片，包括使用碎片离子进行前体离子扫描。

结果

MALDI TOF IMS 分析揭示了iEC 网络中m / z 592.1 和 593.1处的峰的共定位强度。通过 MALDI 成像进行的串联质谱 (MS/MS) 分析，结合使用脂质提取物输注方法的前体离子扫描，确定这些前体离子是溶血磷脂酰胆碱 (LPC) (22:5) 及其同种型。

结论

MALDI 成像分析显示 LPC (22:5) 在 iEC 网络中很丰富。作为疾病和潜在治疗的体外测试模型，目前使用 MALDI 成像的分析方法与例如间充质干细胞 (MSC) 结合到疾病衍生的 iEC 网络可能有助于揭示数量和分布的变化。各种刺激下的脂质。