Differential response of Bruguiera cylindrica to individual (CuSO₄) and combined (CuSO₄ NaCl) effect was evaluated. The plantlets were treated with control, 0.15 mM CuSO₄, 400 mM NaCl and 0.15 mM CuSO₄ + 400 mM NaCl. Under combined stress, higher accumulation of Cu in the roots indicate that the roots are the primary site of Cu accumulation and thus the plant perform as an excluder and photosynthetic efficiency reduced drastically and significant enhancement in the superoxide and hydroxyl free radicals which increase membrane lipid peroxidation, leading to cellular damage and destruction. As evidenced from SEM-EDXMA, increase in Cu and Na⁺ levels in xylem and pith regions of leaf and stem and the presence of deeply stained structures, denoting the probable formation of complex containing the metal. Increased CaOx crystal forming cells (crystal idioblasts) reveals the regulation of bioaccumulated Cu and Na⁺ by complexing with CaOx. Thus the study suggested that, 400 mM NaCl and 0.15 mM CuSO₄ treatments does not have negative impact on plant growth, the NaCl tolerance potential compromised in the presence of mild CuSO₄ concentration during combined stress.

评估了布鲁氏菌对个体（CuSO ₄）和组合（CuSO ₄ NaCl）效应的差异反应。将小植株与对照处理的，0.15毫的CuSO ₄，400mM的NaCl和0.15毫的CuSO ₄  + 400mM的NaCl中。在复合胁迫下，根中较高的铜积累表明根是铜积累的主要部位，因此植物起排斥作用，光合效率急剧降低，超氧化物和羟基自由基显着增强，从而增加了膜脂过氧化作用，导致细胞受损和破坏。从SEM-EDXMA可以看出，Cu和Na ^+的增加茎和叶的木质部和髓区域的水平升高，并且存在深度染色的结构，这表明可能形成了含金属的复合物。CaOx晶体形成细胞（晶体成胚细胞）增加，表明与CaOx络合可调节生物积累的Cu和Na ⁺。因此，该研究表明，400 mM NaCl和0.15 mM CuSO ₄处理对植物生长没有负面影响，在组合胁迫期间，如果存在适度的CuSO ₄浓度，NaCl的耐性可能会降低。