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Isolation and identification of potential probiotic Lactobacillus species from feces of infants in southwest Iran
International Journal of Infectious Diseases ( IF 4.8 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.ijid.2020.05.034
Nabi Jomehzadeh 1 , Hazhir Javaherizadeh 2 , Mansour Amin 3 , Morteza Saki 4 , Mushtak T S Al-Ouqaili 5 , Hajar Hamidi 6 , Maryam Seyedmahmoudi 6 , Zahra Gorjian 7
Affiliation  

OBJECTIVES To evaluate the potential probiotic properties ofLactobacillus strains isolated from feces of infants and also to determine their antimicrobial activity against some enteropathogenic bacteria. METHODS The Fecal samples were prepared from 120 infants aged less than 24 months. In total,105 Lactobacillus strains were identified by phenotypic tests. Thirty isolates were randomly selected to study their potential probiotic properties. These isolates were examined for resistance to acid (pH: 2.5, 2 h) and bile (oxgall 0.3%, 8 h), adhesion to HT-29 cells, antibiotic susceptibility, and antimicrobial activities. RESULTS On basis of 16S rRNA sequencing, 30 isolates identified as Lactobacillus fermentum (n = 11; 36.7%), Lactobacillus plantarum (n = 9; 30%), Lactobacillus rhamnosus (n = 6; 20%), and Lactobacillus paracasei (n = 4; 13.3%). All tested strains survived at acid and bile conditions. Six Lactobacillus strains revealed high adherence to HT-29 cells. Three strains including the L. fermentum (N2, N7), and the L. plantarum (N20) showed good probiotic potential and inhibited the growth of Yersinia enterocolitica ATCC 23715, Shigella flexneri ATCC 12022, Salmonella enterica ATCC 9270, and enteropathogenic Escherichia coli (EPEC) ATCC 43887. The antibiotic resistance test showed that all the isolates were susceptible to tetracycline, and chloramphenicol. CONCLUSIONS Lactobacillus strains like L. fermentum (N2, N7), and the L. plantarum (N20), could be potential probiotic, but further in vitro and in vivo studies on these probiotic strains are still required.

中文翻译:

伊朗西南部婴儿粪便中潜在益生菌乳酸菌的分离与鉴定

目的 评估从婴儿粪便中分离的乳杆菌菌株的潜在益生菌特性,并确定它们对某些肠道致病菌的抗菌活性。方法 粪便样本是从 120 名小于 24 个月的婴儿中制备的。通过表型试验共鉴定出105株乳杆菌。随机选择 30 个分离株来研究它们的潜在益生菌特性。检查这些分离株对酸(pH:2.5,2 小时)和胆汁(牛胆汁 0.3%,8 小时)的抗性、对 HT-29 细胞的粘附性、抗生素敏感性和抗菌活性。结果 根据 16S rRNA 测序,30 个分离株被鉴定为发酵乳杆菌(n = 11;36.7%)、植物乳杆菌(n = 9;30%)、鼠李糖乳杆菌(n = 6;20%)和副干酪乳杆菌( = 4;13.3%)。所有测试菌株在酸性和胆汁条件下均存活。六个乳酸杆菌菌株显示出对 HT-29 细胞的高度粘附。包括发酵乳杆菌 (N2、N7) 和植物乳杆菌 (N20) 在内的三种菌株显示出良好的益生菌潜力,并抑制小肠结肠炎耶尔森氏菌 ATCC 23715、弗氏志贺菌 ATCC 12022、沙门氏菌 ATCC 9270 和肠致病性大肠杆菌(大肠杆菌)的生长。 EPEC) ATCC 43887。抗生素耐药性试验表明,所有分离株均对四环素和氯霉素敏感。结论 发酵乳杆菌 (N2, N7) 和植物乳杆菌 (N20) 等乳杆菌菌株可能是潜在的益生菌,但仍需要对这些益生菌菌株进行进一步的体外和体内研究。包括发酵乳杆菌 (N2、N7) 和植物乳杆菌 (N20) 在内的三种菌株显示出良好的益生菌潜力,并抑制小肠结肠炎耶尔森氏菌 ATCC 23715、弗氏志贺氏菌 ATCC 12022、沙门氏菌 ATCC 9270 和肠致病性大肠杆菌(大肠杆菌)的生长。 EPEC) ATCC 43887。抗生素耐药性试验表明,所有分离株均对四环素和氯霉素敏感。结论 发酵乳杆菌 (N2, N7) 和植物乳杆菌 (N20) 等乳杆菌菌株可能是潜在的益生菌,但仍需要对这些益生菌菌株进行进一步的体外和体内研究。包括发酵乳杆菌 (N2、N7) 和植物乳杆菌 (N20) 在内的三种菌株显示出良好的益生菌潜力,并抑制小肠结肠炎耶尔森氏菌 ATCC 23715、弗氏志贺菌 ATCC 12022、沙门氏菌 ATCC 9270 和肠致病性大肠杆菌(大肠杆菌)的生长。 EPEC) ATCC 43887。抗生素耐药性试验表明,所有分离株均对四环素和氯霉素敏感。结论 发酵乳杆菌 (N2, N7) 和植物乳杆菌 (N20) 等乳杆菌菌株可能是潜在的益生菌,但仍需要对这些益生菌菌株进行进一步的体外和体内研究。和肠致病性大肠杆菌(EPEC)ATCC 43887。抗生素耐药性试验表明所有分离株对四环素和氯霉素敏感。结论 发酵乳杆菌 (N2, N7) 和植物乳杆菌 (N20) 等乳杆菌菌株可能是潜在的益生菌,但仍需要对这些益生菌菌株进行进一步的体外和体内研究。和肠致病性大肠杆菌(EPEC)ATCC 43887。抗生素耐药性试验表明所有分离株对四环素和氯霉素敏感。结论 发酵乳杆菌 (N2, N7) 和植物乳杆菌 (N20) 等乳杆菌菌株可能是潜在的益生菌,但仍需要对这些益生菌菌株进行进一步的体外和体内研究。
更新日期:2020-07-01
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