BACKGROUND This study determined the possible anti-inflammatory and antioxidant renal protective effect of genistein, a soy isoflavone, against kidney damage and functional disorders following renal ischemia/reperfusion (I/R) in male rats. MATERIALS AND METHODS The animals were dedicated to five groups (n=7 per group): Sham, Sham+Geni (genistein, 15 mg/kg in 1 ml 1% DMSO, i.p.), Sham+DMSO (1 ml 1% DMSO, i.p.), I/R (bilateral renal ischemia for 45 min followed by 24 h reperfusion), I/R+Geni (genistein, 15 mg/kg). 24-h urine samples, blood and tissue samples of the kidney were collected at the end of 24 h reperfusion period. RESULTS Compared to sham, sham+Geni and sham+DMSO groups, IR injury (IRI) ended in kidney dysfunction (decreased creatinine clearance, and increased fractional excretion of sodium), increased levels of malondialdehyde, decreased activities of antioxidant enzymes (superoxide dismutase, gluthatione peroxidase, and catalase), increased gene expression levels of TLR4 (Toll-like receptor 4) and TNF-α (tumor necrosis factor-alpha), as well as histological damages in kidney tissue. Genistein administration decreased all the changes. Therefore, genistein apparently protects the kidney against IRI by mitigating both oxidative stress and inflammation. The antioxidant and anti-inflammatory properties of genistein probably exert important roles in improving functional disorders and offer renal protection against IRI.

中文翻译：

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金雀异黄素对缺血性急性肾损伤大鼠模型的保护作用。

背景技术这项研究确定了大豆异黄酮染料木黄酮对雄性大鼠肾脏缺血/再灌注（I / R）后肾损害和功能障碍的抗炎和抗氧化肾脏保护作用。材料与方法将动物分为5组（每组n = 7）：Sham，Sham + Geni（染料木黄酮，在1 ml 1％DMSO中腹膜内注射15 mg / kg，ip），Sham + DMSO（1 ml 1％DMSO， ip），I / R（双侧肾缺血45分钟，然后再灌注24 h），I / R + Geni（染料木黄酮，15 mg / kg）。在24小时再灌注期结束时收集24小时尿液样本，肾脏的血液和组织样本。结果与假，假+ Geni和假+ DMSO组相比，IR损伤（IRI）以肾功能不全（肌酐清除率降低和钠分数排泄增加），丙二醛水平增加，降低抗氧化酶（超氧化物歧化酶，谷胱甘肽过氧化物酶和过氧化氢酶）的活性，增加TLR4（Toll样受体4）和TNF-α（肿瘤坏死因子-α）的基因表达水平，以及肾脏组织的组织学损伤。Genistein管理减少了所有更改。因此，染料木黄酮显然可以通过减轻氧化应激和炎症来保护肾脏抵抗IRI。金雀异黄素的抗氧化和抗炎特性可能在改善功能性疾病中发挥重要作用，并提供针对IRI的肾脏保护。Genistein管理减少了所有更改。因此，染料木黄酮显然可以通过减轻氧化应激和炎症来保护肾脏抵抗IRI。金雀异黄素的抗氧化和抗炎特性可能在改善功能性疾病中发挥重要作用，并提供针对IRI的肾脏保护。Genistein管理减少了所有更改。因此，染料木黄酮显然可以通过减轻氧化应激和炎症来保护肾脏抵抗IRI。金雀异黄素的抗氧化和抗炎特性可能在改善功能性疾病中发挥重要作用，并提供针对IRI的肾脏保护。