Biofloc technology (BFT) is a new green culture technology that is intended not merely to eradicate nitrogenous residues but also enhance immunity and antioxidant activity in aquatic animals. A 56-day feeding trial and a 96 h ammonia challenge test were implemented to evaluate the effect of acute exposure to ammonia and BFT alterations on Rhynchocypris lagowski: digestive enzyme, inflammation response, oxidative stress and immunological parameters in zero water exchange tanks. According to the differences of C/N ratios, the experiment was divided into four groups: C/N 10.8:1 (control group), C/N 15:1, C/N 20: 1 and C/N 25:1. The results provided evidence that weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) were significantly elevated in C/N 20, while food conversion rate (FCR) was considerably decreased (P < 0.05) compared to the control. Significant increases in digestive enzyme amylase (AMS), lipase (LPS), protease (PRS) and cellulase (CES) activity; Immune enzyme complement C3, complement C4, Nitric Oxide Synthase (NOS) and immunoglobulin M (IgM) activity; Serum biochemical lysozyme (LSZ), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (AKP) activity; Inflammation TNF-α, IL-1β, IL-2, IL-6 of R. lagowskis were found in the C/N 20 group after a 56-day feeding trial and a challenging trial (P < 0.05). Comparing the antioxidant capacity of R. lagowski in gills, brains and spleen of juveniles from the four experimental groups, the activity of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT) activity and glutathion reductases (GR) activity of juveniles in the C/N 20 group were significant higher (P < 0.05), and the content of malondialdehyde (MDA) was considerably lower than that in the control. Overall, these findings suggest that BFT not only can enhance R. lagowski growth performance, digestive enzyme activity, and strengthen antioxidant status but also upgrade immune response, improve the ability of resistance to ammonia stress.

Biofloc技术（BFT）是一种新的绿色养殖技术，其目的不仅是消除氮残留，还可以增强水生动物的免疫力和抗氧化活性。进行了一项为期56天的喂养试验和一个96小时的氨激发试验，以评估急性暴露于氨和BFT改变对Rhynchocypris lagowski的影响：零水交换罐中的消化酶，炎症反应，氧化应激和免疫学参数。根据C / N比的差异，将实验分为四组：C / N 10.8：1（对照组），C / N 15：1，C / N 20：1和C / N 25：1。结果提供了证据，表明C / N 20中体重增加率（WGR），比生长率（SGR）和蛋白质效率比（PER）显着提高，而食物转化率（FCR）则显着降低（P<0.05）。消化酶淀粉酶（AMS），脂肪酶（LPS），蛋白酶（PRS）和纤维素酶（CES）活性显着增加；免疫酶补体C3，补体C4，一氧化氮合酶（NOS）和免疫球蛋白M（IgM）活性；血清生化溶菌酶（LSZ），谷氨酸丙酮酸转氨酶（GPT），谷草草酰乙酸转氨酶（GOT）和碱性磷酸酶（AKP）活性；经过56天的喂养试验和具有挑战性的试验，在C / N 20组中发现了R. lagowskis的炎症TNF-α，IL-1β，IL-2，IL-6 （P <0.05）。拉格夫斯基的抗氧化能力比较在四个实验组的幼鱼的brain，脑和脾中，C / N 20组明显高于对照组（P <0.05），丙二醛（MDA）含量明显低于对照组。总体而言，这些发现表明，BFT不仅可以增强拉格夫草的生长性能，消化酶活性并增强抗氧化剂状态，还可以提高免疫反应，提高对氨胁迫的抵抗能力。