Abstract In this study, we present a novel approach to prepare of a colorimetric chemical sensor for H2O2 and a glucose biosensor basing on the use of peroxidase-like activity of silver nanoparticles decorated on reduced graphene oxide sheets (AgNPs@rGO) nanocomposite. Herein, AgNPs@rGO nanocomposite was synthesized by a one-step hydrothermal reducing method and its physico-chemical properties were characterized by X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), Ultraviolet–visible spectroscopy (UV–Vis), Fourier-Transform Infrared spectroscopy (FT-IR) and Energy Dispersive X-ray spectroscopy (EDX). Obtained evaluation results shown that the synthesized AgNPs/rGO nanocomposite has performed an efficient peroxidase-like activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMBred) by H2O2, leading to the oxidized form (TMBox) which presents a typical blue color (maximum of absorbance at λmax = 655 nm). A colorimetric assay for H2O2 detection was designed and fabricated with a limit of detection of 20 μM. Moreover, we have used of AgNPs/rGO nanocomposite combining with glucose oxidase (GOx) to develop of a colorimetric glucose biosensor with a low limit of detection of 40 μM and a linear dynamic range from 125 μM to 1 mM. This glucose test was applied to the detection of glucose in human serum samples.

中文翻译：

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银纳米颗粒装饰的还原氧化石墨烯：一种用于开发比色葡萄糖生物传感器的新型过氧化物酶样活性纳米材料

摘要 在这项研究中，我们提出了一种新的方法来制备 H2O2 比色化学传感器和葡萄糖生物传感器，该方法基于使用装饰在还原氧化石墨烯片 (AgNPs@rGO) 纳米复合材料上的银纳米颗粒的过氧化物酶样活性。在此，AgNPs@rGO 纳米复合材料通过一步水热还原法合成，其理化性质通过 X 射线衍射 (XRD)、透射电子显微镜 (TEM)、紫外-可见光谱 (UV-Vis)、傅里叶变换红外光谱 (FT-IR) 和能量色散 X 射线光谱 (EDX)。获得的评估结果表明，合成的 AgNPs/rGO 纳米复合材料对 H2O2 氧化 3,3',5,5'-四甲基联苯胺（TMBred）具有有效的过氧化物酶样活性，导致氧化形式 (TMBox)，呈现典型的蓝色（λmax = 655 nm 处的最大吸光度）。设计并制造了一种检测 H2O2 的比色法，检测限为 20 μM。此外，我们使用 AgNPs/rGO 纳米复合材料与葡萄糖氧化酶 (GOx) 结合开发了一种比色葡萄糖生物传感器，其检测下限为 40 μM，线性动态范围为 125 μM 至 1 mM。该葡萄糖测试用于检测人血清样品中的葡萄糖。我们使用 AgNPs/rGO 纳米复合材料与葡萄糖氧化酶 (GOx) 结合开发了一种比色葡萄糖生物传感器，其检测下限为 40 μM，线性动态范围为 125 μM 至 1 mM。该葡萄糖测试用于检测人血清样品中的葡萄糖。我们使用 AgNPs/rGO 纳米复合材料与葡萄糖氧化酶 (GOx) 结合开发了一种比色葡萄糖生物传感器，其检测下限为 40 μM，线性动态范围为 125 μM 至 1 mM。该葡萄糖测试用于检测人血清样品中的葡萄糖。