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Molecular detection of Euphorbia yellow mosaic virus infecting chili pepper
Tropical Plant Pathology ( IF 1.5 ) Pub Date : 2020-05-18 , DOI: 10.1007/s40858-020-00365-6
Aricléia de M. Catarino , Thiago F. Sousa , Emilly J. S. P. de Lima , Francisco M. Zerbini , Osvaldo F. L. Sande , Monique B. Nascimento , Jeferson C. da Cruz , Rogério E. Hanada , Áquila R. do Nascimento , Luiz A. G. de Assis , Cristovão A. da Costa , Gilvan F. da Silva

Begomoviruses (family Geminiviridae, genus Begomovirus) are responsible for considerable losses in several tropical crops, more commonly in bean and tomato. Begomoviruses comprise the most numerous and economically destructive group within Geminiviridae. Characteristic symptoms of begomovirus infection in Capsicum chinense (chili pepper, Solanaceae) were observed in smallholder farms in the state of Amazonas. The molecular detection and identification of begomoviruses affecting chili pepper were conducted from symptomatic foliar samples collected at 14 farms in the municipalities of Presidente Figueiredo, Iranduba and Manaus (Amazonas state). Begomovirus was detected in five out of the 69 samples, all of the cultivar Lupita. Pairwise sequence comparisons and phylogenetic analysis based on the complete sequence of the DNA-A component identified the isolates as members of the species Euphorbia yellow mosaic virus (EuYMV). Comparative analysis with 36 EuYMV isolates grouped the Amazonian isolates in a single clade, indicating a low degree of genetic variability for this begomovirus.

中文翻译:

大戟黄花叶病毒侵染辣椒的分子检测

Begomoviruses(双生病毒科,Begomovirus 属)是导致几种热带作物大量损失的原因,在豆类和番茄中更常见。Begomoviruses 是 Geminiviridae 中数量最多、最具经济破坏性的类群。在亚马逊州的小农农场中观察到了辣椒(辣椒,茄科)中 begomovirus 感染的特征性症状。从菲格雷多总统、伊兰杜巴和马瑙斯(亚马逊州)市的 14 个农场收集的有症状的叶面样本中对影响辣椒的 begomovirus 进行了分子检测和鉴定。在 69 个样本中的 5 个样本中检测到 Begomovirus,所有样本均为栽培品种 Lupita。基于 DNA-A 组分的完整序列的成对序列比较和系统发育分析将分离物鉴定为大戟黄花叶病毒 (EuYMV) 物种的成员。与 36 个 EuYMV 分离株的比较分析将亚马逊分离株归为一个进化枝,表明该 begomovirus 的遗传变异程度较低。
更新日期:2020-05-18
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