Using a novel curcumin-loaded niosome nanoparticle (CM-NP), the present study was designed to evaluate the effect of curcumin on human glioblastoma stem-like cells (GSCs). CM-NP has a diameter of ~ 60 nm and a zeta potential of ~ - 35 mV with a constant physicochemical stability. The cytotoxic effects of free curcumin (CM) and CM-NP were investigated on GSCs obtained during the removal of a brain tumor. Both CM and CM-NP caused a dose-dependent decrease in cell proliferation and viability of GSCs. The IC50 values of CM and CM-NP on GSCs were 50 and 137 μg/ml after 24 h, respectively. CM-NP exerted significantly higher effects on GSC viability, apoptosis, cell cycle arrest, and the expression of Bax, a pro-apoptotic marker, compared with CM. In addition, the migration of GSCs was significantly impaired following the administration of CM-NP compared with CM. Furthermore, CM-NP significantly increased the values of reactive oxygen species and decreased the mRNA expressions of NF-κB and IL-6 of GSCs compared with CM. Our data also revealed that CM-NP could significantly reduce the invasiveness of GSCs compared with CM, possibly via MCP-1-mediated pathways. In addition, CM-NP exhibited a significantly greater inhibitory effect on colony formation of GSCs compared with CM. These data indicate that CM-NP exhibited stronger anti-tumor effects on GSCs than CM. Although further in vivo investigations are warranted, our results suggest that CM-NP could be an ideal carrier to deliver curcumin for potential therapeutic approaches into glioblastoma.

中文翻译：

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脂质体纳米颗粒中负载的姜黄素改善了游离姜黄素对胶质母细胞瘤干细胞样细胞的抗肿瘤作用：一项体外研究。

本研究使用新型姜黄素负载的脂质体纳米颗粒（CM-NP）来评估姜黄素对人胶质母细胞瘤干细胞（GSC）的影响。CM-NP 的直径约为 60 nm，zeta 电位约为 35 mV，具有恒定的物理化学稳定性。研究人员研究了游离姜黄素 (CM) 和 CM-NP 对脑肿瘤切除过程中获得的 GSC 的细胞毒性作用。CM 和 CM-NP 均导致 GSC 的细胞增殖和活力呈剂量依赖性下降。24小时后，CM和CM-NP对GSC的IC50值分别为50和137 μg/ml。与 CM 相比，CM-NP 对 GSC 活力、细胞凋亡、细胞周期停滞和促凋亡标记 Bax 的表达具有显着更高的影响。此外，与 CM 相比，施用 CM-NP 后 GSC 的迁移显着受损。此外，与CM相比，CM-NP显着增加GSCs活性氧的值并降低NF-κB和IL-6的mRNA表达。我们的数据还显示，与 CM 相比，CM-NP 可以显着降低 GSC 的侵袭性，这可能是通过 MCP-1 介导的途径。此外，与CM相比，CM-NP对GSC集落形成的抑制作用明显更强。这些数据表明，CM-NP 对 GSC 表现出比 CM 更强的抗肿瘤作用。尽管需要进一步的体内研究，但我们的结果表明，CM-NP 可能是传递姜黄素的理想载体，用于胶质母细胞瘤的潜在治疗方法。