Sex-specific DNA markers are very helpful for identifying genetic sex and studying sex determination mechanisms in fish. To identify the sex-specific markers of spotted knifejaw (Oplegnathus punctatus), we performed a comparative analysis of the female and male genomes. In this study, an 18 bp insertion was identified in the male genome after verification by sequencing depth and PCR. An effective and rapid method based on PCR was then developed to identify the genetic sex. A male-female-shared primer pair and a male-specific primer were designed for PCR amplification to avoid false-negative phenomena. To examine the primers in practice, we utilized hundreds of spotted knifejaw fish from different groups to identify their genetic sex, and the results were consistent with their phenotypic sex. The male-specific DNA marker would be helpful for artificial breeding, Y chromosome assembly and further study of the sex determination mechanism. This study is the first to identify an effective sex-specific marker in spotted knifejaw.

中文翻译：

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斑点刀颚 (Oplegnathus punctatus) 雄性特异性分子标记的分离和遗传性别鉴定技术的开发。

性别特异性 DNA 标记对于识别遗传性别和研究鱼类的性别决定机制非常有帮助。确定斑点刀颚（Oplegnathus punctatus ）的性别特异性标记)，我们对女性和男性基因组进行了比较分析。在这项研究中，经过测序深度和 PCR 验证后，在雄性基因组中发现了 18 bp 的插入。然后开发了一种基于 PCR 的有效且快速的方法来识别遗传性别。设计了雄性-雌性共用引物对和雄性特异性引物用于 PCR 扩增，以避免假阴性现象。为了在实践中检查引物，我们利用来自不同群体的数百条斑点刀颚鱼来确定它们的遗传性别，结果与它们的表型性别一致。雄性特异性DNA标记将有助于人工育种、Y染色体组装和性别决定机制的进一步研究。这项研究是第一个在斑点刀颚中确定有效的性别特异性标记物的研究。