当前位置: X-MOL 学术Int. Ophthalmol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods.
International Ophthalmology ( IF 1.4 ) Pub Date : 2020-05-18 , DOI: 10.1007/s10792-020-01393-6
Jasem Saki 1 , Elham Eskandari 2 , Mostafa Feghhi 3
Affiliation  

INTRODUCTION Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debilitating vision loss.In Khuzestan province, southwest of Iran, T. gondii infection has been reported to be significant but toxocariasis was rare. However, the frequency of ocular toxoplasmosis and toxocariasis has not been studied in this area. The aim of this study was to evaluate the ocular toxoplasmosis and ocular toxocariasis using serological and molecular methods. METHOD In this case control study, 310 patients were identified by ophthalmologist as ocular toxoplasmosis and then 5 cc of venous blood samples were taken from each of them. Serum samples and buffy coat were prepared and ELISA was used to detect IgG and IgM anti-Toxoplasma antibodies and the molecular PCR was used to detect Toxoplasma DNA parasite in buffy coats. ELISA test was used to detect of IgG anti-Toxocara antibodies. RESULTS Totally, for ocular toxoplasmosis, 130 (41.93%) of 310 patients were positive by ELISA, of them 121 (39%) IgG positive and nine (2.9%) IgM positive were diagnosed. Of 121 cases with IgG+, 119 (98.35%) were diagnosed with high IgG avidity indicating chronic phase of the infection. For ocular toxocariasis evaluation, antibodies against Toxocara were not detected in any of the samples. By PCR molecular method, 11 out of 310 patients (3.54%) had T. gondii DNA in the blood. In control, in total, 21 cases were detected positive by serology method, which showed a significant difference with the results of the case group(P < 0.05).By PCR method, only three cases showed positive which also indicated significant difference with result of case group (3 vs 9) (P < 0.05). In the control group, also no anti-toxocara antibodies were found. CONCLUSION It can be concluded that T. gondii in Khuzestan province as the etiologic agent of ocular toxoplasmosis and physicians should consider diagnostic methods for identifying the infection when they visit the patients.

中文翻译:

使用血清学和分子方法研究患有眼科疾病的患者的弓形虫病和弓形虫病。

简介弓形虫是一种细胞内的原生动物寄生虫,可引起眼部弓形虫病,并伴有大多数并发症,例如视网膜脱离。在猫和狗中发现的弓形虫弓形虫是人的幼虫,会引起严重的眼部并发症,例如使视力下降。在伊朗西南部的胡泽斯坦省,据报道弓形虫感染很严重,但弓形虫病很少见。然而,尚未在该领域研究眼弓形虫病和弓形虫病的频率。这项研究的目的是使用血清学和分子方法评估眼弓形虫病和眼弓形虫病。方法在本病例对照研究中,眼科医生将310例患者确定为眼弓形体病,然后从每个患者中抽取5 cc静脉血样本。制备血清样品和血沉棕黄层,使用ELISA检测IgG和IgM抗弓形虫抗体,并使用分子PCR检测血沉棕黄层中的弓形虫DNA寄生虫。ELISA试验用于检测IgG抗弓形虫抗体。结果总体而言,眼部弓形虫病的310例患者中有130例(41.93%)ELISA呈阳性,其中121例(39%)IgG阳性和9例(2.9%)IgM阳性被诊断。在121例IgG +病例中,有119例(98.35%)被诊断出高IgG亲和力,表明感染呈慢性。对于眼弓形虫病评估,在任何样品中均未检测到针对弓形虫的抗体。通过PCR分子方法,在310名患者中有11名(3.54%)的血液中存在弓形虫DNA。在对照中,通过血清学方法总共检测到21例阳性,与病例组比较,差异有统计学意义(P <0.05)。PCR法仅3例阳性,与病例组比较差异有统计学意义(3 vs 9)(P <0.05)。在对照组中,也未发现抗毒素的抗体。结论可以得出结论,胡泽斯坦省的弓形虫是眼弓形体病的病原体,因此,医生在探视患者时应考虑使用诊断方法来鉴定感染。
更新日期:2020-05-18
down
wechat
bug