The influence of non-thermal plasma (NTP) treatment on the prevention of antibiotic resistance of microbial biofilms was studied. Staphylococcus epidermidis and Escherichia coli bacteria and a yeast Candida albicans, grown on the surface of Ti-6Al-4V alloy used in the manufacture of prosthetic implants, were employed. Their biofilms were exposed to NTP produced by DC cometary discharge and subsequently treated with antibiotics commonly used for the treatment of infections caused by them: erythromycin (ERY), polymyxin B (PMB), or amphotericin B (AMB), respectively. All biofilms displayed significant reduction of their metabolic activity after NTP exposure, the most sensitive was S. epidermidis. The subsequent action of antibiotics caused significant decrease in the metabolic activity of S. epidermidis and E. coli, but not C. albicans, although the area covered by biofilm decreased in all cases. The combined effect of NTP with antibiotics was thus proved to be a promising strategy in bacterial pathogen treatment.

中文翻译：

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非热血浆和随后的抗生素治疗相结合，可防止生物膜的再发育。

研究了非热等离子体（NTP）处理对预防微生物生物膜的抗生素耐药性的影响。使用表皮葡萄球菌和大肠杆菌细菌以及白色假丝酵母菌，其生长在用于制造假体的Ti-6Al-4V合金表面上。它们的生物膜暴露于DC彗星放电产生的NTP，然后分别用通常用于治疗由它们引起的感染的抗生素进行治疗：红霉素（ERY），多粘菌素B（PMB）或两性霉素B（AMB）。NTP暴露后，所有生物膜均显示其代谢活性显着降低，最敏感的是表皮葡萄球菌。抗生素的后续作用导致表皮葡萄球菌和大肠杆菌的代谢活性大大降低，但白色念珠菌却没有，尽管在所有情况下，被生物膜覆盖的面积都在减少。因此，NTP与抗生素的联合作用被证明是细菌病原体治疗中的一种有前途的策略。