当前位置: X-MOL 学术Cell Tissue Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Restoration of estrous cycles by co-transplantation of mouse ovarian tissue with MSCs
Cell and Tissue Research ( IF 3.2 ) Pub Date : 2020-05-19 , DOI: 10.1007/s00441-020-03204-x
Zohreh Mehdinia 1 , Mahnaz Ashrafi 2 , Rouhollah Fathi 3 , Payam Taheri 4 , Mojtaba Rezazadeh Valojerdi 3, 5
Affiliation  

This study investigates the effect of bone marrow (BM-MSCs) and visceral peritoneum (VP-MSCs)-derived mesenchymal stem cells on the transplanted ovary. VP-MSCs and BM-MSCs were obtained from green fluorescent protein-expressing mice (GFP+). Six- to eight-week-old female NMRI mice were divided into four experimental groups, autograft ovarian tissue fragments (AO), autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel (AO-H), autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel containing BM-MSCs (AO-HB) and autograft ovarian tissue fragments encapsulated in fibrin-collagen hydrogel containing VP-MSCs (AO-HP). Intact ovary (IO) was the control group. The estrous cycles resumption time was monitored and at the third estrous cycle, the blood samples and grafted ovaries were evaluated using hormonal, histological and gene expression analysis. Onset of estrous cycles, especially at the second cycle, was earlier in AO-HB and AO-HP groups than in the AO-H group (P < 0.05). Moreover, E2 and FSH levels in AO-HB and AO-HP groups were returned to those of the intact group. However, folliculogenesis was still retarded as compared with the IO group. The gene expression of theca (Lhcgr, Cyp17a1, Gli2, Gli3 and Ptch1), granulosa (Amh and Fshr), oocyte (Zp3 and Gdf9), germ cells (Stella and Prdm1), angiogenesis (VEGF and bFGF) and apoptosis (Bax/Bcl2 and Caspase3) markers was similar in both AO-HB and AO-HP groups. Expression of Amh, Fshr, Gdf9 and VEGF increased only in the AO-HP group whereas expression of Ptch1 increased only in the AO-HB group, as compared with the AO group (P < 0.05). In conclusion, BM-MSCs or VP-MSCs can improve ovarian autotransplantation in mice with no superiority over each other.

中文翻译:

小鼠卵巢组织与间充质干细胞联合移植恢复发情周期

本研究调查骨髓 (BM-MSCs) 和内脏腹膜 (VP-MSCs) 来源的间充质干细胞对移植卵巢的影响。VP-MSCs 和 BM-MSCs 来自表达绿色荧光蛋白的小鼠 (GFP+)。将 6 至 8 周龄的雌性 NMRI 小鼠分为四个实验组,自体卵巢组织碎片(AO)、包裹在纤维蛋白 - 胶原水凝胶(AO-H)中的自体卵巢组织碎片、包裹在纤维蛋白中的自体卵巢组织碎片 -含有 BM-MSC (AO-HB) 的胶原水凝胶和包裹在含有 VP-MSC (AO-HP) 的纤维蛋白-胶原水凝胶中的自体移植卵巢组织碎片。完整卵巢(IO)是对照组。监测发情周期恢复时间,并在第三个发情周期,使用激素评估血样和移植卵巢,组织学和基因表达分析。AO-HB 和 AO-HP 组发情周期的开始,特别是在第二个周期,早于 AO-H 组(P < 0.05)。此外,AO-HB 和 AO-HP 组的 E2 和 FSH 水平恢复到完整组的水平。然而,与 IO 组相比,卵泡生成仍然延迟。鞘膜(Lhcgr、Cyp17a1、Gli2、Gli3 和 Ptch1)、颗粒(Amh 和 Fshr)、卵母细胞(Zp3 和 Gdf9)、生殖细胞(Stella 和 Prdm1)、血管生成(VEGF 和 bFGF)和细胞凋亡(Bax/ Bcl2 和 Caspase3) 标记在 AO-HB 和 AO-HP 组中相似。Amh、Fshr、Gdf9 和 VEGF 的表达仅在 AO-HP 组中增加,而 Ptch1 的表达仅在 AO-HB 组中增加,与 AO 组相比(P < 0.05)。综上所述,
更新日期:2020-05-19
down
wechat
bug