Aim The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration. Results Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ ) gene expression in MSCs treated group showed significant increase compared to diabetic group ( p value < 0.001 ) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups ( p value < 0.001 ). Histopathological and immune - histochemical examination revealed regeneration of pancreatic islets in both treated groups. Conclusion MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves

中文翻译：

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间充质干细胞来源的外泌体治疗诱导性1型糖尿病大鼠的作用

目的 本研究的目的是评估 MSC 衍生的外泌体在治疗 1 型 DM 中的治疗和再生效果，并将其效果与 MSC 本身进行比较。实验在 40 只白化大鼠上进行，分组如下，组（1）：10 只健康大鼠，组（2）：10 只诱导 1 型 DM 大鼠，组（3）：10 只诱导 1 型 DM 大鼠腹膜内接受外泌体，组(4)：10只诱导的1型DM大鼠腹膜内接受MSC。每周评估血清葡萄糖和血浆胰岛素水平。进行 QRT-PCR 以通过测量胰岛素、Pdx1、Smad2、Smad3 和 TGFβ 基因来评估胰腺 β 细胞的再生。此外，还进行了组织病理学和免疫组织化学检查以确认胰腺组织再生。结果 关于评估的基因（胰岛素、Pdx1、Smad2、Smad3和Tgfβ)基因表达与糖尿病组相比显着增加(p值<0.001)，外泌体基因表达与糖尿病和MSCs治疗组相比显着增加(p值<0.001)。组织病理学和免疫-组织化学检查揭示了两个治疗组中胰岛的再生。结论 MSCs 衍生的外泌体显示出比 MSCs 本身更好的治疗和再生效果 组织病理学和免疫-组织化学检查揭示了两个治疗组中胰岛的再生。结论 MSCs 衍生的外泌体显示出比 MSCs 本身更好的治疗和再生效果 组织病理学和免疫-组织化学检查揭示了两个治疗组中胰岛的再生。结论 MSCs 衍生的外泌体显示出比 MSCs 本身更好的治疗和再生效果