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In vivo and in vitro assessment of Trichoderma species and Bacillus thuringiensis integration to mitigate insect pests of brinjal (Solanum melongena L.)
Egyptian Journal of Biological Pest Control ( IF 2.1 ) Pub Date : 2020-05-18 , DOI: 10.1186/s41938-020-00258-5
Ahmad Nawaz , Muhammad Dildar Gogi , Muhammad Naveed , Muhammad Arshad , Muhammad Sufyan , Muhammad Binyameen , Shams Ul Islam , Muhammad Waseem , Muhammad Bilal Ayyub , Muhammad Jalal Arif , Habib Ali

The present study was performed to assess the individual and combined potential of 3 different concentrations of Trichoderma sp. (Ts) and Bacillus thuringiensis (Bt) to control brinjal insect pests. Tested formulations were applied on larval and adult stages of the pest. The Trichoderma laboratory bioassays revealed 73% mortality of the aphid species, Aphis gossypii (Glover), and 53% mortality of the cotton leafhopper (Jassids) Amrasca bigutulla bigutulla (Ishida), while opposite results were observed in case of Bt at the highest concentration (1 × 108 cfu ml−1) used. In vivo results revealed that Ts caused a significant population reduction of the aphid (87%) than the jassid (72%), 7 days of post-treatment, at the highest concentration, while non-significant results were observed at the lowest concentrations. A. gossypii was significantly found more susceptible to the mixture of Trichoderma + Bt than the jassid (62%) and brinjal shoot and fruit borer (65%) even after 48 h of treatment application. The combined application (Trichoderma + Bt) showed maximum population reduction of jassid (88%), aphid (95%), and BSFB (96%), respectively, 7 days post-applications. The positive correlation among time and concentration was observed. The result may imply that consortium of these microbial organisms could be effective and can be incorporated in IPM programs for effective control of sucking and chewing insect pests of brinjal.

中文翻译:

在体内和体外评估木霉物种和苏云金芽孢杆菌的整合,以减轻昆虫茄子的害虫(茄子L.

进行本研究以评估3种不同浓度的木霉菌的个体潜力和综合潜力。(Ts)和苏云金芽孢杆菌(Bt)来控制茄子害虫。将经测试的制剂应用于害虫的幼虫和成年阶段。木霉属实验室的生物测定显示,蚜虫物种棉蚜(Glover)的死亡率为73%,棉叶蝉(Jassids)Amrasca bigutulla bigutulla(Ishida)的死亡率为53%,而Bt浓度最高时观察到相反的结果。使用(1×108 cfu ml-1)。体内结果显示,在最高浓度的处理后7天,Ts引起的蚜虫种群数量显着减少,低于Jaassid种群(72%),而在最低浓度下未观察到显着的结果。一种。甚至在施药48小时后,发现棉酚比木霉菌+ Bt混合物更容易受到木霉菌+ Bt的侵害,而不是jassid和茄子和果bri(65%)。联合施用(木霉菌+ Bt)在施用后7天分别显示出最大的种群减少量为jassid(88%),蚜虫(95%)和BSFB(96%)。观察到时间和浓度之间的正相关。结果可能暗示这些微生物有机体的联盟可能是有效的,并且可以纳入IPM计划中,以有效控制茄子的吸吮和咀嚼害虫。联合施用(木霉菌+ Bt)在施用后7天分别显示出最大的种群减少量为jassid(88%),蚜虫(95%)和BSFB(96%)。观察到时间和浓度之间的正相关。结果可能暗示这些微生物有机体的联盟可能是有效的,并且可以纳入IPM计划中,以有效控制茄子的吸吮和咀嚼害虫。联合施用(木霉菌+ Bt)在施用后7天分别显示出最大的种群减少量为jassid(88%),蚜虫(95%)和BSFB(96%)。观察到时间和浓度之间的正相关。结果可能暗示这些微生物有机体的联盟可能是有效的,并且可以纳入IPM计划中,以有效控制茄子的吸吮和咀嚼害虫。
更新日期:2020-05-18
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