Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. Here, we present tappAS, the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation. tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of transcript variants and isoform regulation. tappAS software and documentation are available at https://app.tappas.org .

中文翻译：

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tappAS：用于分析差异剪接功能影响的综合计算框架


长读长测序的最新进展解决了短读长 RNA-Seq 数据中全长转录本的替代转录本识别的不准确性，这鼓励了以异构体为中心的功能分析方法的开发。在这里，我们提出了tappAS，这是第一个能够进行全面的功能同转录组学（FIT）分析的框架，它可以有效揭示特定背景转录后调控的功能影响。 tappAS 使用编码和非编码功能域、基序和位点的异构体解析注释，结合新颖的分析方法来询问转录变体和异构体调节的功能读数的不同方面。 TappAS 软件和文档可在 https://app.tappas.org 上获取。