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Self-aligned 3D microlenses in a chip fabricated with two-photon stereolithography for highly sensitive absorbance measurement.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-05-18 , DOI: 10.1039/d0lc00235f Jiukai Tang 1 , Guangyu Qiu , Xiaobao Cao , Yang Yue , Xiaole Zhang , Jean Schmitt , Jing Wang
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-05-18 , DOI: 10.1039/d0lc00235f Jiukai Tang 1 , Guangyu Qiu , Xiaobao Cao , Yang Yue , Xiaole Zhang , Jean Schmitt , Jing Wang
Affiliation
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Absorbance measurement is a widely used method to quantify the concentration of an analyte. The integration of absorbance analysis in microfluidic chips could significantly reduce the sample consumption and contribute to the system miniaturization. However, the sensitivity and limit of detection (LoD) of analysis in microfluidic chips with conventional configuration need improvements due to the limited optical pathway and unregulated light propagation. In this work, a 3D-microlens-incorporating microfluidic chip (3D-MIMC) with a greatly extended detection channel was innovatively fabricated using two-photon stereolithography. The fabrication was optimized with a proposed hierarchical modular printing strategy. Due to the incorporation of 3D microlenses, the light coupling efficiency and the signal-to-noise ratio (SNR) were respectively improved approximately 9 and 4 times. An equivalent optical path length (EOL) of 62.9 mm was achieved in a 3.7 μl detection channel for testing tartrazine samples. As a result, the sensitivity and LoD of the 3D-MIMC assay were correspondingly improved by one order of magnitude, compared with those of the 96-well plate assay. Notably, the 3D-MIMC has the potential to be integrated into a general microanalysis platform for multiple applications.
中文翻译：
采用双光子立体光刻技术制造的芯片中的自对准3D微透镜，用于高度敏感的吸光度测量。
吸光度测量是一种广泛用于量化分析物浓度的方法。在微流控芯片中集成吸光度分析可以显着减少样品消耗并有助于系统小型化。然而，由于光路受限和光传播不受限制，具有常规配置的微流体芯片的分析灵敏度和检测限（LoD）需要改进。在这项工作中，使用双光子立体光刻技术创新地制造了带有3D-微透镜的微流控芯片（3D-MIMC），该芯片具有大大扩展的检测通道。通过提出的分层模块化印刷策略优化了制造。由于加入了3D微透镜，光耦合效率和信噪比（SNR）分别提高了约9倍和4倍。在用于测试酒石detection样品的3.7μl检测通道中，等效光程长度（EOL）为62.9 mm。结果，与96孔板测定法相比，3D-MIMC测定法的灵敏度和LoD相应地提高了一个数量级。值得注意的是，3D-MIMC有潜力集成到通用的微分析平台中，以用于多种应用。
更新日期：2020-06-30
中文翻译：
采用双光子立体光刻技术制造的芯片中的自对准3D微透镜，用于高度敏感的吸光度测量。
吸光度测量是一种广泛用于量化分析物浓度的方法。在微流控芯片中集成吸光度分析可以显着减少样品消耗并有助于系统小型化。然而，由于光路受限和光传播不受限制，具有常规配置的微流体芯片的分析灵敏度和检测限（LoD）需要改进。在这项工作中，使用双光子立体光刻技术创新地制造了带有3D-微透镜的微流控芯片（3D-MIMC），该芯片具有大大扩展的检测通道。通过提出的分层模块化印刷策略优化了制造。由于加入了3D微透镜，光耦合效率和信噪比（SNR）分别提高了约9倍和4倍。在用于测试酒石detection样品的3.7μl检测通道中，等效光程长度（EOL）为62.9 mm。结果，与96孔板测定法相比，3D-MIMC测定法的灵敏度和LoD相应地提高了一个数量级。值得注意的是，3D-MIMC有潜力集成到通用的微分析平台中，以用于多种应用。
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