BACKGROUND Gastric cancer is one of the most common malignancies worldwide with high mortality. Therefore, identifying cancer-related biomarkers for predicting prognosis and progression of gastric cancer is essential. This study aimed to investigate the clinical value and functional role of microRNA-3196 in gastric cancer. METHODS The relative expression levels of microRNA-3196 in gastric cancer tissues and adjacent normal tissues were detected by quantitative reverse transcription-polymerase chain reaction. In this study, quantitative reverse transcription-polymerase chain reaction, cell proliferation assay, and Transwell migration and invasion assays were performed to explore microRNA-3196 expression level and its effects on cell proliferation, migration, and invasion in gastric cancer cells. The Kaplan-Meier method and multivariate Cox regression analyses were used to explore the prognostic significance of microRNA-3196 in gastric cancer. Dual-luciferase report assay was performed to validate the potential target gene regulated by microRNA-3196 in gastric cancer. RESULTS The expression of microRNA-3196 was downregulated in gastric cancer tissues and cell lines. Downregulation of microRNA-3196 was associated with lymph node metastasis and Tumor Node Metastasis (TNM) stage. The Kaplan-Meier curve analysis indicated that patients with low expression of microRNA-3196 had a poor prognosis, and the Cox regression analysis results showed microRNA-3196 expression was an independent prognostic factor of gastric cancer. Moreover, overexpression of microRNA-3196 inhibited cell proliferation, migration, and invasion, while knockdown of microRNA-3196 promoted these cellular behaviors in AGS and MKN45 cells. OTX1 may be a potential target gene regulated by microRNA-3196 in gastric cancer. CONCLUSIONS These results suggested that microRNA-3196 might not only a tumor suppressor in gastric cancer cells by modulating OTX1 but also might be an independent prognostic biomarker and therapeutic target of gastric cancer.

中文翻译：

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miR-3196充当肿瘤抑制因子并预测胃癌患者的生存结果。

背景技术胃癌是全世界死亡率最高的最常见的恶性肿瘤之一。因此，鉴定与癌症相关的生物标志物以预测胃癌的预后和进展至关重要。本研究旨在探讨microRNA-3196在胃癌中的临床价值和功能作用。方法采用定量逆转录-聚合酶链反应检测胃癌组织及癌旁正常组织中microRNA-3196的相对表达水平。在这项研究中，进行了定量逆转录聚合酶链反应，细胞增殖测定以及Transwell迁移和侵袭测定，以探讨microRNA-3196表达水平及其对胃癌细胞中细胞增殖，迁移和侵袭的影响。Kaplan-Meier方法和多因素Cox回归分析用于探讨microRNA-3196在胃癌中的预后意义。进行了双重荧光素酶报告检测，以验证microRNA-3196调控胃癌的潜在靶基因。结果在胃癌组织和细胞株中microRNA-3196的表达下调。microRNA-3196的下调与淋巴结转移和肿瘤转移（TNM）阶段有关。Kaplan-Meier曲线分析表明，microRNA-3196表达低的患者预后较差，Cox回归分析结果表明microRNA-3196表达是胃癌的独立预后因素。此外，microRNA-3196的过表达抑制细胞增殖，迁移和侵袭，而敲低microRNA-3196则促进了AGS和MKN45细胞的这些细胞行为。OTX1可能是microRNA-3196在胃癌中调控的潜在靶基因。结论这些结果表明，microRNA-3196不仅可以通过调节OTX1抑制胃癌细胞的抑癌作用，而且可能是胃癌的独立预后生物标志物和治疗靶标。