Fucoidan Immobilized at the Surface of a Fibrous Mesh Presents Toxic Effects over Melanoma Cells, But Not over Noncancer Skin Cells.,Biomacromolecules

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Fucoidan Immobilized at the Surface of a Fibrous Mesh Presents Toxic Effects over Melanoma Cells, But Not over Noncancer Skin Cells.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-05-18 , DOI: 10.1021/acs.biomac.0c00482
Catarina Oliveira _{1,

2} , Ana I Soares _{1,

2} , Nuno M Neves _{1,

2,

3} , Rui L Reis _{1,

2,

3} , Alexandra P Marques _{1,

2,

3} , Tiago H Silva _{1,

2} , Albino Martins _{1,

2}

Affiliation

The use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mL^–1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment.

中文翻译：

固定在纤维网表面的岩藻依聚糖对黑素瘤细胞具有毒性作用，但对非癌性皮肤细胞无毒性作用。

本文提出将岩藻依聚糖（一种海洋来源的生物活性聚合物）的使用作为抗黑素瘤（最具侵袭性的皮肤癌之一）的创新且有效策略的组成部分。首先，与人类皮肤成纤维细胞和角质形成细胞相比，评估了岩藻依聚糖的可溶形式的抗肿瘤活性，对黑素瘤细胞具有增强的细胞毒性。经过这种抗肿瘤活性的验证并试图开发出一种旨在减少针对非癌细胞的细胞毒性作用的更具针对性和局部性的策略后，将岩藻依聚糖固定在电纺纳米纤维网（NFM_Fu）的表面，以期开发一种治疗性贴剂。最大固定浓度为1.2 mg mL ^–1通过甲苯胺蓝测定法测定并通过XPS确认。此外，NFM_Fu比NFM更具亲水性，接触角为36°，低于控制条件的121°。NFM_Fu能够将人类黑素瘤细胞活力降低50％，而不会影响人类皮肤成纤维细胞和角质形成细胞。综上所述，这些结果为黑色素瘤治疗的宝贵方法奠定了基础。

更新日期：2020-07-13

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