Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing on different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus (LCD22, LCD25, LCK22 and LCK25), Ensifer meliloti (LCK8, LCK9 and LCK12), and Mesorhizobium camelthorni (LCD11, LCD13, LCD31 and LCD33). In addition, phylogenetic analyses revealed eight additional strains with previously undescribed chromosomal lineages within the genera Ensifer (LCF5, LCF6 and LCF8), Rhizobium (LCF11, LCF12 and LCF14) and Mesorhizobium (LCF16 and LCF19). Analysis using nodC gene identified five symbiovar groups, four of which were already known. The remaining group composed of two strains (LCD11 and LCD33) represented a new symbiovar of Mesorhizobium camelthorni, which we propose designating as sv. hedysari. Interestingly, we report that soil properties drive and structure the symbiosis of L. creticus and its rhizobia.

中文翻译：

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具有不同共生体的新型推定的根瘤菌根瘤状莲Lotus瘤和它们对独特土壤特性的差异偏爱。

从突尼斯莲花的共生结节中分离出突尼斯特有的系统发育上不同的根瘤菌菌株，它们生长在突尼斯的不同极端极端微生物地理区域上，并使用多系统进化分析技术进行了鉴定，如华氏根瘤菌（LCK33，LCK35，LCO42和LCO49），Ensifer ，LCD25，LCK22和LCK25），半球形的Ensifer meliloti（LCK8，LCK9和LCK12）和骆驼中生根瘤菌（LCD11，LCD13，LCD31和LCD33）。此外，系统发育分析还显示了Ensifer属（LCF5，LCF6和LCF8），根瘤菌属（LCF11，LCF12和LCF14）和中生根瘤菌（LCF16和LCF19）内的八种其他菌株，它们具有以前未描述的染色体谱系。使用nodC基因进行的分析确定了五个共生体组，其中四个是已知的。剩下的由两个菌株（LCD11和LCD33）组成的组代表了一种新的共生骆驼科共生体，我们建议将其命名为sv。Hedysari。有趣的是，我们报道了土壤特性驱动和构建了小etic草及其根瘤菌的共生关系。