Agricultural biotechnology strategies often require the precise regulation of multiple genes to effectively modify complex plant traits. However, most efforts are hindered by a lack of characterized tools that allow for reliable and targeted expression of transgenes. We have successfully engineered a library of synthetic transcriptional regulators that modulate expression strength in planta. By leveraging orthogonal regulatory systems from Saccharomyces spp., we have developed a strategy for the design of synthetic activators, synthetic repressors, and synthetic promoters and have validated their use in Nicotiana benthamiana and Arabidopsis thaliana. This characterization of contributing genetic elements that dictate gene expression represents a foundation for the rational design of refined synthetic regulators. Our findings demonstrate that these tools provide variation in transcriptional output while enabling the concerted expression of multiple genes in a tissue-specific and environmentally responsive manner, providing a basis for generating complex genetic circuits that process endogenous and environmental stimuli.

中文翻译：

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用于调节植物基因表达的正交调节系统的设计。


农业生物技术策略通常需要精确调控多个基因，以有效改变复杂的植物性状。然而，大多数努力都因缺乏能够可靠且有针对性地表达转基因的特征工具而受到阻碍。我们成功地设计了一个合成转录调节子文库，可以调节植物中的表达强度。通过利用酵母属的正交调控系统，我们开发了一种设计合成激活剂、合成阻遏物和合成启动子的策略，并验证了它们在本塞姆氏烟草和拟南芥中的应用。这种决定基因表达的贡献遗传元件的特征为合理设计精制合成调节剂奠定了基础。我们的研究结果表明，这些工具提供了转录输出的变化，同时能够以组织特异性和环境响应的方式协调表达多个基因，为生成处理内源性和环境刺激的复杂遗传电路提供了基础。