In this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so reducing sampling volumes and improving operation convenience. Amino-functionalized graphene (NH₂-GO)/thionine (THI)/gold particle (AuNP) nanocomposites were used to modify the working electrode not only to generate the electrochemical signals, but also to provide an environment conducive to aptamer immobilization. Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode, thus resulting in a lower signal. The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL⁻¹ (R² = 0.989) and a detection limit of 5 pgmL⁻¹ for EGFR. The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples, showing good agreement with the gold-standard enzyme-linked immunosorbent assay.

在这项工作中，通过采用抗 EGFR 适配体作为生物识别元件，制造了一种电化学纸基适配体传感器，用于表皮生长因子受体 (EGFR) 的无标记和超灵敏检测。该装置利用折纸或折纸的概念，作为进样和检测之间的阀门，减少了进样量，提高了操作便利性。氨基功能化石墨烯 (NH ₂-GO）/硫氨酸（THI）/金颗粒（AuNP）纳米复合材料用于修饰工作电极，不仅可以产生电化学信号，还可以提供有利于适配体固定的环境。电化学表征表明，绝缘适体-抗原免疫复合物的形成会阻碍电子从样品介质转移到工作电极，从而导致较低的信号。实验结果表明，所提出的适体传感器的线性范围为 0.05 至 200 ngmL ^-1 ( R ²  = 0.989)，检测限为 5 pgmL ^-1对于 EGFR。通过分析血清样品进一步研究了所提出的纸质适体传感器的分析可靠性，表明与金标准酶联免疫吸附测定法具有良好的一致性。