N⁶-2-hydroxyethyl-adenosine (HEA) is one of the main bioactive components found in Cordyceps cicadae and it has been reported to display antioxidant and anti-inflammatory activities. Cisplatin (CP) is one of the most commonly used chemotherapeutic drug for treating various cancers and tumors, but the use is widely curtailed due to its toxicity of various organs including the kidney. This study was aimed at investigating the protective effect of HEA on cisplatin-induced kidney injury. Mice were pretreated with HEA for 7 days and administered with cisplatin. Kidney function index including blood urea nitrogen (BUN), creatinine, renal oxidative stress and pro-inflammatory indices such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), tumor necrosis factor (TNF-α), interleukin 1 beta (IL-1β) and interleukin 6 (IL-6) were measured. Histopathological assessment of the kidney was also performed. The results indicated that HEA noticeably modulated the levels of BUN and creatinine as well as decreased the expression of MDA, TNF-α, IL-1β and IL-6 in the kidney. In addition, HEA up regulated the activities of antioxidant enzymes SOD, CAT and GSH-Px. Therefore, we concluded that HEA could effectively alleviate cisplatin-induced nephrotoxicity by counteracting oxidative stress and inflammation.

N ⁶ -2-羟乙基腺苷（HEA）是在冬虫夏草中发现的主要生物活性成分之一据报道它具有抗氧化和消炎的作用。顺铂（CP）是用于治疗各种癌症和肿瘤的最常用化学治疗药物之一，但由于其对包括肾脏在内的各种器官的毒性而被广泛限制使用。这项研究旨在调查HEA对顺铂诱导的肾脏损伤的保护作用。小鼠用HEA预处理7天，并用顺铂给药。肾脏功能指数包括血液尿素氮（BUN），肌酐，肾氧化应激和促炎指数，例如丙二醛（MDA），超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GSH-Px），肿瘤坏死因子测定了TNF-α，白介素1β（IL-1β）和白介素6（IL-6）。还进行了肾脏的组织病理学评估。结果表明，HEA明显调节了肾脏中BUN和肌酐的水平，并降低了MDA，TNF-α，IL-1β和IL-6的表达。此外，HEA上调了抗氧化酶SOD，CAT和GSH-Px的活性。因此，我们得出的结论是，HEA可通过抵消氧化应激和炎症反应来有效减轻顺铂引起的肾毒性。