Abstract

With the increase of antibiotic resistance, which is present at a worrying rate, research on the use of newly developed nanoparticles as an antimicrobial agent with green biotechnology has intensified. The study aimed to investigate the antimicrobial effects of chitosan nanoparticles (CSNP) synthesized using Pleurotus eryngii extract (PE). Characterization of P. eryngii-loaded chitosan nanoparticles (PE-CSNPs) was performed with Fourier transform infrared spectrophotometer, X-ray diffraction, Field-emission scanning electron microscopy, Brunauer–Emmett–Teller, Differential scanning calorimetry, and zeta potential techniques. The FE-SEM images showed that the surface morphology of nanoparticles is similar to CS, but has more porosity network and smaller dimensions structure. The average particle size of spherical PE-CSNPs was obtained as 330.1 nm. The specific surface area and average pore diameter of the synthesized nanoparticles were found as 3.99 m²g⁻¹ and 2.25 nm, respectively. X-ray diffraction determines the presence of an amorphous peak at 2θ = 21.2° results from CS and PE. PE-CSNPs synthesized using P. eryngii extract showed strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans as 0.0156, 0.0625, 0.0625 and 0.0312 mg ml⁻¹, respectively. Thus, it was determined that chitosan nanoparticles formed by the green synthesis of P. eryngii extract showed strong anti-microbial properties.

摘要

随着以令人担忧的速度出现的对抗生素的抗性增加，通过绿色生物技术对使用新开发的纳米颗粒作为抗微生物剂的研究已经加强。该研究旨在研究使用杏鲍菇提取物（PE）合成的壳聚糖纳米颗粒（CSNP）的抗菌作用。表征杏鲍菇傅里叶变换红外分光光度计，X射线衍射，场发射扫描电子显微镜，Brunauer-Emmett-Teller，差示扫描量热法和zeta电位技术进行了负载壳聚糖纳米颗粒（PE-CSNPs）的分析。FE-SEM图像表明，纳米粒子的表面形貌与CS相似，但具有更大的孔隙网络和较小的尺寸结构。球形PE-CSNP的平均粒径为330.1 nm。发现合成的纳米颗粒的比表面积和平均孔径分别为3.99m ² g ^-1和2.25nm。X射线衍射确定在2的非晶峰的存在θ 从CS和PE = 21.2°的结果。PE-CSNPs合成使用杏鲍菇提取物显示出对大肠杆菌，金黄色葡萄球菌，枯草芽孢杆菌和白色念珠菌的强抗菌活性，分别为0.0156、0.0625、0.0625和0.0312 mg ml ^-1。因此，确定了通过绿脓杆菌提取物的绿色合成形成的壳聚糖纳米颗粒显示出强的抗微生物特性。