Abstract

Sequencing analysis of mitochondrial genomes is useful for understanding the genome structures. In this study, complete mitochondrial genomes of the Eospalax smithii was obtained by using next generation sequencing method. The complete mitogenome of E. smithii was 16,350 bp long, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 1 non-coding control region (D-loop). The overall base composition of the heavy strand is A (33.65%), C (23.80%), T (30.31%), and G (12.24%). The base compositions present highly biased toward A + T nucleotides. The result of phylogenetic analysis showed the five Eospalax species formed a monophyly with the high bootstrap value and as a sister group of the genus Myospalax. This is the first report of the complete mitochondrial genomes of E. smithii and the mitogenome is potentially important for evolutionary biology, population genetics, and species diagnosis studies of the Mysopalacinae.

摘要

线粒体基因组的测序分析对于理解基因组结构很有用。在这项研究中，通过使用下一代测序方法获得了Eospalax smithii的完整线粒体基因组。的完整线粒体基因E.史密斯是16,350个碱基长，含有13蛋白编码基因（个PCG），2核糖体RNA（rRNA基因）基因，22转移RNA（tRNA）的基因，和1个非编码控制区（d-环） 。重链的总体基本组成为A（33.65％），C（23.80％），T（30.31％）和G（12.24％）。基础组合物呈现出高度偏向A + T核苷酸的趋势。系统发育分析结果表明，这5种os草属具有较高的自举值，并作为属的姊妹群。肌pal。这是史密斯肠杆菌完整线粒体基因组的首次报道，而有丝分裂基因组对于Mysopalacinae的进化生物学，种群遗传学和物种诊断研究可能具有重要意义。