Abstract We prepared CdSe QDs on thioglycolic acid modified chitosan (CdSe@Chit-TGAc). It showed bright yellow fluorescence under long wave UV radiation. Optical properties and growth of crystal was checked by ultraviolet-visible spectroscopy and photoluminescence spectroscopy. HRTEM and XPS results demonstrated that CdSe QDs were successfully prepared and decorated on thiol modified chitosan templet and size of CdSe QDs was also successfully stabilized within the quantum dot size range. Biocompatibility of CdSe@Chit-TGAc was checked on in-vitro system in two cell lines i.e., normal lung cell line (WI-38) and pulmonary cancerous cell line (A549) which exhibited significant uptake and florescence of CdSe@Chit-TGAc with WI-38. The concentration-dependent cytotoxicity in A549 cells for the QDs was also studied. Based on preliminary bioassays, further mechanistic studies can be done to exploit the properties of CdSe@Chit-TGAc as fluorescent agent for cell imaging, targeted drug delivery, nano-medicine etc. GRAPHICAL ABSTRACT

中文翻译：

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硫乙醇酸修饰的壳聚糖：原位合成用于细胞成像的 CdSe QD 的模板

摘要 我们在巯基乙酸修饰的壳聚糖 (CdSe@Chit-TGAc) 上制备了 CdSe QD。它在长波紫外线辐射下呈亮黄色荧光。通过紫外可见光谱和光致发光光谱检查晶体的光学性质和生长。HRTEM 和 XPS 结果表明，CdSe QDs 成功制备并装饰在硫醇改性壳聚糖模板上，CdSe QDs 的尺寸也成功地稳定在量子点尺寸范围内。CdSe@Chit-TGAc 的生物相容性在两种细胞系即正常肺细胞系 (WI-38) 和肺癌细胞系 (A549) 的体外系统上进行了检查，它们表现出显着的 CdSe@Chit-TGAc 吸收和荧光WI-38。还研究了 A549 细胞中 QD 的浓度依赖性细胞毒性。根据初步生物测定，