ABSTRACT

The aim of this study was to develop a highly-sensitive liquid chromatographic – tandem mass spectrometric (LC-MS/MS) method to investigate the presence of aflatoxins (AFB₁, AFB₂, AFG₁ and AFG₂) and ochratoxin A (OTA) in traditional homemade sausages (n = 88) collected from small family farms situated in different regions in Croatia. Aflatoxins were not detected (<LOD) in any of the analysed samples, whereas the percentage of OTA-positive homemade sausages was 14.8%, consisting of 1.1% Istrian sausages, 7.9% Slavonian sausages and 5.7% Kulenova Seka. In Slavonian sausages, the mean OTA concentration was 0.27 ± 0.09 μg/kg with a maximum of 0.38 μg/kg, while in Kulenova Seka the mean concentration was 0.26 ± 0.14 μg/kg, rising to a maximum of 0.48 μg/kg. Statistically significant differences (p = .243) in OTA concentrations across different types of homemade sausage and sampling regions were not determined.

摘要

这项研究的目的是开发一种高灵敏度液相色谱-串联质谱（LC-MS / MS）方法，以研究黄曲霉毒素（AFB ₁，AFB ₂，AFG ₁和AFG _{2）的存在}）和从克罗地亚不同地区的小型家庭农场收集的传统自制香肠（n = 88）中的ra曲霉毒素A（OTA）。在所有分析样品中均未检出黄曲霉毒素（<LOD），而OTA阳性自制香肠的百分比为14.8％，其中包括1.1％的伊斯特拉香肠，7.9％的斯拉沃尼亚香肠和5.7％的Kulenova Seka。在Slavonian香肠中，平均OTA浓度为0.27±0.09μg/ kg，最大为0.38μg/ kg，而在Kulenova Seka中，平均OTA浓度为0.26±0.14μg/ kg，最大为0.48μg/ kg。尚未确定不同类型的自制香肠和采样区域中OTA浓度的统计显着差异（p = .243）。