ABSTRACT

This study investigated the effect of carbon sources (n = 2) on the performance of a microbial community in an anaerobic moving-bed biofilm reactor (MBBR) treating acid mine drainage (AMD). The 1.5 L anaerobic MBBR was operated across a range of hydraulic retention times – HRT’s (3–18 days), using different substrates, i.e. brewing wastewater and lactate as sole carbon sources and electron donors. Maximum sulphate reduction and chemical oxygen demand (COD) consumption rate was 21.94 and 24.28 mg ${\mathrm{SO}}_4^{2-}$ L⁻¹ h⁻¹, and 0.473 and 0.697 mg COD L⁻¹ d⁻¹ for brewing wastewater and lactate supplemented bioreactors, respectively, at an HRT of 3 days. The maximum COD/${\mathrm{SO}}_4^{2-}$ ratio was found to be 2.564 in the bioreactor supplemented with brewing wastewater at an HRT of 15 days. The metal removal above 70% in the system supplemented with brewing wastewater followed the order; Be²⁺ > Fe²⁺ > Sr²⁺ > Pb²⁺ > Mg²⁺ > Cu²⁺ > Zn²⁺ > Li¹⁺ > Ca²⁺ in comparison to the system supplemented with lactate, Be²⁺ > Fe²⁺ > Sr²⁺ > Mg²⁺ > Cu²⁺ > Li¹⁺ > Zn²⁺ > Pb²⁺ after an HRT of 18 days. Complete removal of beryllium (II) was observed irrespective of the carbon source used. The results clearly showed that brewing wastewater can be deployed as a nutritional supplement in environmental remediation of AMD.

摘要

本研究调查了碳源 ( n  = 2) 对处理酸性矿山排水 (AMD) 的厌氧移动床生物膜反应器 (MBBR) 中微生物群落性能的影响。1.5 L 厌氧 MBBR 在一系列水力停留时间 - HRT（3-18 天）内运行，使用不同的底物，即酿造废水和乳酸作为唯一的碳源和电子供体。最大硫酸盐还原和化学需氧量 (COD) 消耗率为 21.94 和 24.28 毫克${\mathrm{所以}}_4^{2-}$L ^-1 h ^-1以及0.473 和0.697 mg COD L ^-1 d ^{-1 分别}用于酿造废水和补充乳酸的生物反应器，HRT 为3 天。最大COD/${\mathrm{所以}}_4^{2-}$在 HRT 为 15 天的情况下，在补充有酿造废水的生物反应器中发现该比率为 2.564。补充酿造废水的系统金属去除率70%以上依次递减；Be ²⁺  > Fe ²⁺  > Sr ²⁺  > Pb ²⁺  > Mg ²⁺  > Cu ²⁺  > Zn ²⁺  > Li ¹⁺  > Ca ²⁺与添加乳酸的系统相比，Be ²⁺  > Fe ^{2 +}  > Sr ²⁺  > Mg ²⁺  > Cu ²⁺  > Li ¹⁺  > Zn ²⁺  > Pb ²⁺经过 18 天的 HRT。无论使用何种碳源，均观察到铍 (II) 的完全去除。结果清楚地表明，酿造废水可以作为AMD环境修复的营养补充剂。